Reduced long-term hematopoietic reconstitution potential of expanded cells that are cycling at transplantation
| . | G0/G1 . | S/G2/M . | P Value . |
|---|---|---|---|
| 1° Recipients, n: | 15 | 6 | |
| Total leukocytes | 77 ± 5 | 39 ± 14 | .04 |
| B cells | 87 ± 5 | 48 ± 15 | .05 |
| T cells | 62 ± 4 | 29 ± 11 | .03 |
| Myeloid cells | 65 ± 7 | 22 ± 11 | .007 |
| 2° Recipients, n: | 9 | 5 | |
| Total leukocytes | 52 ± 8 | 15 ± 12 | .04 |
| B cells | 66 ± 8 | 21 ± 16 | .05 |
| T cells | 39 ± 5 | 14 ± 10 | .06 |
| Myeloid cells | 40 ± 12 | 22 ± 17 | .04 |
| . | G0/G1 . | S/G2/M . | P Value . |
|---|---|---|---|
| 1° Recipients, n: | 15 | 6 | |
| Total leukocytes | 77 ± 5 | 39 ± 14 | .04 |
| B cells | 87 ± 5 | 48 ± 15 | .05 |
| T cells | 62 ± 4 | 29 ± 11 | .03 |
| Myeloid cells | 65 ± 7 | 22 ± 11 | .007 |
| 2° Recipients, n: | 9 | 5 | |
| Total leukocytes | 52 ± 8 | 15 ± 12 | .04 |
| B cells | 66 ± 8 | 21 ± 16 | .05 |
| T cells | 39 ± 5 | 14 ± 10 | .06 |
| Myeloid cells | 40 ± 12 | 22 ± 17 | .04 |
The expanded progeny of Sca-1+c-kit+Lin− BM cells (Ly-5.2+) were labeled with HO dye, sorted into G0/G1 or S/G2/M fractions, and injected into lethally irradiated Ly-5.1 mice (106cells/mouse). The data shown are the mean ± SEM percentage of Ly-5.2+ blood cells measured 4 months later (pooled data from 3 experiments). Primary BM cells were then transplanted into secondary mice (0.5 femur/mouse), which were analyzed after 10 weeks.P values represent comparisons of engraftment by G0/G1 and S/G2/M cells.