Effects of retinoids on properties of cultured mast cells supported by SCF
| Stimuli . | Cell size (μm) . | Histamine content (pg/cell) . | Chymase+ cells (%) . |
|---|---|---|---|
| SCF | 21.5 ± 3.6 | 3.00 ± 0.47 | 33.9 ± 7.5 |
| SCF + ATRA | 13.3 ± 3.3* | 1.44 ± 0.18† | 31.2 ± 8.4 |
| SCF + 9-cis RA | 13.0 ± 3.2* | 1.41 ± 0.10† | 32.8 ± 7.2 |
| SCF + Ro 13-7410 | 13.7 ± 3.6* | 1.47 ± 0.24† | 35.4 ± 5.1 |
| SCF + Ro 25-7386 | 20.1 ± 3.7 | 2.63 ± 0.77 | 34.7 ± 8.1 |
| SCF + IL-4 | 25.0 ± 3.3* | 6.24 ± 0.96* | 72.4 ± 9.1* |
| SCF + IL-6 | 29.7 ± 5.3* | 15.84 ± 2.17* | 82.5 ± 9.8* |
| Stimuli . | Cell size (μm) . | Histamine content (pg/cell) . | Chymase+ cells (%) . |
|---|---|---|---|
| SCF | 21.5 ± 3.6 | 3.00 ± 0.47 | 33.9 ± 7.5 |
| SCF + ATRA | 13.3 ± 3.3* | 1.44 ± 0.18† | 31.2 ± 8.4 |
| SCF + 9-cis RA | 13.0 ± 3.2* | 1.41 ± 0.10† | 32.8 ± 7.2 |
| SCF + Ro 13-7410 | 13.7 ± 3.6* | 1.47 ± 0.24† | 35.4 ± 5.1 |
| SCF + Ro 25-7386 | 20.1 ± 3.7 | 2.63 ± 0.77 | 34.7 ± 8.1 |
| SCF + IL-4 | 25.0 ± 3.3* | 6.24 ± 0.96* | 72.4 ± 9.1* |
| SCF + IL-6 | 29.7 ± 5.3* | 15.84 ± 2.17* | 82.5 ± 9.8* |
SCF = stem cell factor; ATRA = all-trans retinoic acid.
The 1 × 104 10-week cultured mast cells were incubated in culture wells containing SCF, retinoids, IL-6, or IL-4, alone or in combination. After 2 weeks, the cells were processed for immunocytochemical staining with an antitryptase or antichymase MoAb. The diameter of tryptase+ cells was measured, as described in “Materials and methods.” At the same time, intracellular histamine concentrations were determined by the radioimmunoassay. The results shown are the mean ± SD of 4 experiments. SCF, 100 ng/mL; ATRA, 9-cis RA, Ro 13-7410, Ro 25-7386, 10−7mol/L; IL-4, 20 ng/mL; IL-6, 50 ng/mL. Significantly different from SCF alone (*P < .0001,
P < .005).