Immunoglobulin heavy chain gene analysis of follicular lymphomas
| Patient No. . | H Chain Isotype (protein)* . | H Chain Isotype (RNA)* . | VH Family . | Closest Germline Gene . | No. of Mutations (%) . | D Gene . | JH Gene . | Intraclonal Variation‡ (no. of clones sequenced) . |
|---|---|---|---|---|---|---|---|---|
| 1 | IgM1-153 | μ1-153 | VH3 | V3-23 | 12 (4.1) | D2-8 (D1) | JH4b | nd |
| 3-′93 | IgM, IgD | μ, δ | VH3 | V3-7 (VH3-11) | 26 (8.8) | na | JH6b | 0.4 (5) |
| 3-′95 | IgM | μ, δ | VH3 | V3-7 (VH3-11) | 21 (7.1) | na | JH6b | 0.3 (6) |
| 13 | IgM, IgD | μ, δ | VH4 | V4-34 (DP-63) | 9 (3) | na | JH6b | 3.2 (5) |
| 15 | IgM | μ | VH4 | V4-34 (DP-63) | 8 (2.7) | na | JH2 | 0.3 (5) |
| 16 | IgM, IgD | μ, δ | VH3 | V3-11 | 49 (16.7) | na | JH5b | nd |
| 17 | IgM | μ, δ | VH3 | V3-23 | 16 (5.4) | na | JH3b | 0.3 (3)1-155 |
| 18 | IgM, IgD | μ, δ | VH3 | V3-30 (DP-49) | 26 (8.8) | na | JH6c | nd |
| 32 | IgM1-153 | μ, δ | VH7 | V7-4.1 (VI-4.1b) | 26 (8.8) | na | JH3b | nd |
| 57 | IgM | μ, δ | VH3 | V3-7 | 25 (8.5) | D3-22 (D21-9) | JH4b | 0.2 (9) |
| 58 | IgM | μ, δ | VH4 | V4-34 | 26 (8.8) | D2-2 (D4-b) | JH4b | 2.0 (5) |
| 67 | IgM | μ, δ | VH4 | V4-39 (DP-79) | 25 (8.4) | D5-24 | JH4b | nd |
| 8-′83 | IgM | μ, δ | VH3 | V3-23 | 30 (10.2) | D2-15/D2 | JH3b | 5.0 (7) |
| 8-′83 | IgG | γ | VH3 | V3-23 | 35 (11.9) | D2-15/D2 | JH3b | 4.0 (5) |
| 8-′92 | IgG | γ | VH3 | V3-23 | 35 (11.9) | D2-15/D2 | JH3b | 1.3 (6) |
| 2 | IgG | γ | VH3 | V3-30 (DP-49) | 37 (12.6) | na | JH1 | 0.7 (3) |
| 4 | IgG | γ | VH3 | V3-23 | 49 (16.7) | na | JH3a | 14.6 (5)1-160 |
| 5 | IgG | γ | VH3 | V3-30 (COS8) | 20 (6.8) | na | JH4b | nd |
| 6-′94 | IgG | γ | VH3 | V3-23 | 44 (15) | na | JH4b | 2.8 (4) |
| 6-′98 | Unclear | γ | VH3 | V3-23 | 50 (17) | na | JH4b | 1.8 (6) |
| 25 | Unclear | γ | VH3 | V3-30 | 20 (6.8) | na | JH4b | 0.4 (5) |
| 35 | IgG | γ | VH1 | V1-69 (DP-10) | 34 (11.6) | na | JH6b | 1.8 (8) |
| 50 | IgG | γ | VH4 | V4-61 (3d279d) | 51 (17.2) | na | JH5a | nd |
| 52 | IgG | γ | VH3 | V3-7 | 42 (14.3) | na | JH5a | 3.2 (6) |
| 56 | IgM, weak IgG | γ | VH3 | V3-23 | 26 (8.8) | na | JH6c | nd |
| 59 | IgG | γ | VH5 | V5-51 (COS24) | 11 (3.7) | D6-19 | JH6b | 3.8 (4) |
| 66-′96 | IgG | γ | VH4 | V4-39 (DP-79) | 38 (12.8) | na | JH4b | 3.0 (6) |
| 66-′97 | IgG | γ | VH4 | V4-39 (DP-79) | 39 (13.1) | na | JH4b | 0.7 (6) |
| 66-′98 | IgG | γ | VH4 | V4-39 (DP-79) | 40 (13.5) | na | JH4b | 0.5 (6) |
| 68 | IgG | γ | VH3 | V3-30 (DP-49) | 8 (2.7) | na | JH5b | nd |
| 49 | IgA | α | VH1 | V1-18 | 29 (9.9) | na | JH4b | 6.3 (6) |
| Patient No. . | H Chain Isotype (protein)* . | H Chain Isotype (RNA)* . | VH Family . | Closest Germline Gene . | No. of Mutations (%) . | D Gene . | JH Gene . | Intraclonal Variation‡ (no. of clones sequenced) . |
|---|---|---|---|---|---|---|---|---|
| 1 | IgM1-153 | μ1-153 | VH3 | V3-23 | 12 (4.1) | D2-8 (D1) | JH4b | nd |
| 3-′93 | IgM, IgD | μ, δ | VH3 | V3-7 (VH3-11) | 26 (8.8) | na | JH6b | 0.4 (5) |
| 3-′95 | IgM | μ, δ | VH3 | V3-7 (VH3-11) | 21 (7.1) | na | JH6b | 0.3 (6) |
| 13 | IgM, IgD | μ, δ | VH4 | V4-34 (DP-63) | 9 (3) | na | JH6b | 3.2 (5) |
| 15 | IgM | μ | VH4 | V4-34 (DP-63) | 8 (2.7) | na | JH2 | 0.3 (5) |
| 16 | IgM, IgD | μ, δ | VH3 | V3-11 | 49 (16.7) | na | JH5b | nd |
| 17 | IgM | μ, δ | VH3 | V3-23 | 16 (5.4) | na | JH3b | 0.3 (3)1-155 |
| 18 | IgM, IgD | μ, δ | VH3 | V3-30 (DP-49) | 26 (8.8) | na | JH6c | nd |
| 32 | IgM1-153 | μ, δ | VH7 | V7-4.1 (VI-4.1b) | 26 (8.8) | na | JH3b | nd |
| 57 | IgM | μ, δ | VH3 | V3-7 | 25 (8.5) | D3-22 (D21-9) | JH4b | 0.2 (9) |
| 58 | IgM | μ, δ | VH4 | V4-34 | 26 (8.8) | D2-2 (D4-b) | JH4b | 2.0 (5) |
| 67 | IgM | μ, δ | VH4 | V4-39 (DP-79) | 25 (8.4) | D5-24 | JH4b | nd |
| 8-′83 | IgM | μ, δ | VH3 | V3-23 | 30 (10.2) | D2-15/D2 | JH3b | 5.0 (7) |
| 8-′83 | IgG | γ | VH3 | V3-23 | 35 (11.9) | D2-15/D2 | JH3b | 4.0 (5) |
| 8-′92 | IgG | γ | VH3 | V3-23 | 35 (11.9) | D2-15/D2 | JH3b | 1.3 (6) |
| 2 | IgG | γ | VH3 | V3-30 (DP-49) | 37 (12.6) | na | JH1 | 0.7 (3) |
| 4 | IgG | γ | VH3 | V3-23 | 49 (16.7) | na | JH3a | 14.6 (5)1-160 |
| 5 | IgG | γ | VH3 | V3-30 (COS8) | 20 (6.8) | na | JH4b | nd |
| 6-′94 | IgG | γ | VH3 | V3-23 | 44 (15) | na | JH4b | 2.8 (4) |
| 6-′98 | Unclear | γ | VH3 | V3-23 | 50 (17) | na | JH4b | 1.8 (6) |
| 25 | Unclear | γ | VH3 | V3-30 | 20 (6.8) | na | JH4b | 0.4 (5) |
| 35 | IgG | γ | VH1 | V1-69 (DP-10) | 34 (11.6) | na | JH6b | 1.8 (8) |
| 50 | IgG | γ | VH4 | V4-61 (3d279d) | 51 (17.2) | na | JH5a | nd |
| 52 | IgG | γ | VH3 | V3-7 | 42 (14.3) | na | JH5a | 3.2 (6) |
| 56 | IgM, weak IgG | γ | VH3 | V3-23 | 26 (8.8) | na | JH6c | nd |
| 59 | IgG | γ | VH5 | V5-51 (COS24) | 11 (3.7) | D6-19 | JH6b | 3.8 (4) |
| 66-′96 | IgG | γ | VH4 | V4-39 (DP-79) | 38 (12.8) | na | JH4b | 3.0 (6) |
| 66-′97 | IgG | γ | VH4 | V4-39 (DP-79) | 39 (13.1) | na | JH4b | 0.7 (6) |
| 66-′98 | IgG | γ | VH4 | V4-39 (DP-79) | 40 (13.5) | na | JH4b | 0.5 (6) |
| 68 | IgG | γ | VH3 | V3-30 (DP-49) | 8 (2.7) | na | JH5b | nd |
| 49 | IgA | α | VH1 | V1-18 | 29 (9.9) | na | JH4b | 6.3 (6) |
nd indicates not determined; na, the D gene could not definitely be assigned to a germline D gene.
Heavy chain isotype expression as determined immunohistochemically or by PCR.
The intraclonal variation is indicated as the number of mutations observed per clone, compared with the consensus sequence.
IgD expression was not assessed.
One clone was excluded from the analysis, because it carried a deletion of 31 base pairs.
One clone carried 47 mutations compared with the consensus clone.