Direct effects of G-CSF on IFN-γ and IL-4 production by CD4+ T lymphocytes
| Mixture . | G-CSF Exposure . | IFN-γ Concentration . | IL-4 Concentration . |
|---|---|---|---|
| Lymphocytes | No | 300 | 2.4 |
| Monocytes | No | ||
| Lymphocytes | Yes | 100 | 4 |
| Monocytes | No | ||
| Lymphocytes | No | 320 | 2 |
| Monocytes | Yes |
| Mixture . | G-CSF Exposure . | IFN-γ Concentration . | IL-4 Concentration . |
|---|---|---|---|
| Lymphocytes | No | 300 | 2.4 |
| Monocytes | No | ||
| Lymphocytes | Yes | 100 | 4 |
| Monocytes | No | ||
| Lymphocytes | No | 320 | 2 |
| Monocytes | Yes |
Monocytes and CD4+ cells from normal donors were purified to obtain preparations with greater than 98% purity as determined by flow cytometry using CD4 and CD14 mAb. Monocytes and CD4+ cells were separately exposed to G-CSF for 24 hours, washed, and mixed together (ratio 1:1). IFN-γ and IL-4 were measured using ELISA and were expressed as pg/mL.