Release by NK cells of PAF, perforin, and IFN-γ compared with PAF receptor expression on targets and 51Cr release
| Time . | 0 Min . | 5 Min . | 10 Min . | 30 Min . | 60 Min . | 120 Min . | 180 Min . | 240 Min . |
|---|---|---|---|---|---|---|---|---|
| PAF release (pg) | ||||||||
| NK + K562 | 0 | 0 | 0 | 175 | 87.5 | 0 | — | 0 |
| NK + Daudi | 0 | 0 | 62.5 | 250 | 0 | 0 | — | 0 |
| Percentage of BLT esterase | ||||||||
| NK + K562 | 0 | 98 | 54 | 50 | 47 | 45 | 45 | 49 |
| NK + Daudi | 0 | 78 | 44 | 42 | 42 | 38 | 41 | 42 |
| IFN-γ release (pg) | ||||||||
| NK + K562 | 0 | 715 | 645 | 708 | 733 | 730 | — | 780 |
| NK + Daudi | 0 | 716 | 707 | 721 | 727 | 729 | — | 714 |
| Percentage of target PAF-receptor expression | ||||||||
| NK + K562 | 0 | — | 33 | 23 | — | — | — | — |
| NK + Daudi | 0 | — | 0 | 1 | — | — | — | — |
| Percentage of 51Cr release | ||||||||
| NK + K562 | — | — | 5 | 5 | 11 | 21 | 30 | 29 |
| NK + Daudi | — | — | 0 | 0 | 0 | 4 | 6 | 4 |
| Time . | 0 Min . | 5 Min . | 10 Min . | 30 Min . | 60 Min . | 120 Min . | 180 Min . | 240 Min . |
|---|---|---|---|---|---|---|---|---|
| PAF release (pg) | ||||||||
| NK + K562 | 0 | 0 | 0 | 175 | 87.5 | 0 | — | 0 |
| NK + Daudi | 0 | 0 | 62.5 | 250 | 0 | 0 | — | 0 |
| Percentage of BLT esterase | ||||||||
| NK + K562 | 0 | 98 | 54 | 50 | 47 | 45 | 45 | 49 |
| NK + Daudi | 0 | 78 | 44 | 42 | 42 | 38 | 41 | 42 |
| IFN-γ release (pg) | ||||||||
| NK + K562 | 0 | 715 | 645 | 708 | 733 | 730 | — | 780 |
| NK + Daudi | 0 | 716 | 707 | 721 | 727 | 729 | — | 714 |
| Percentage of target PAF-receptor expression | ||||||||
| NK + K562 | 0 | — | 33 | 23 | — | — | — | — |
| NK + Daudi | 0 | — | 0 | 1 | — | — | — | — |
| Percentage of 51Cr release | ||||||||
| NK + K562 | — | — | 5 | 5 | 11 | 21 | 30 | 29 |
| NK + Daudi | — | — | 0 | 0 | 0 | 4 | 6 | 4 |