Hematopoietic chimerism in NOD/Lt mice given varying sublethal doses of radiation and C57BL/6 bone marrow in the presence or absence of anti-CD154 monoclonal antibody
| Radiation (rad) . | Anti-CD154 mAb . | Number of Chimeric Mice (%) . | Percentage of Donor-Origin PBMCs in Chimeric Mice . | |
|---|---|---|---|---|
| Number of Injections . | Dose . | |||
| 600 | 0 | — | 0/6 (0%) | — |
| 14 | 0.25 mg | 3/6 (50%) | 24%, 93%, >99% | |
| 2 | 0.5 mg | 8/12 (67%) | 95%, 97%, Six >99% | |
| 700 | 0 | — | 4/4 (100%) | All >99% |
| 14 | 0.25 mg | 1/3 (33%) | All >99% | |
| 2 | 0.5 mg | 5/6 (83%) | All >99% | |
| 800 | 0 | — | 2/2 (100%) | Both >99% |
| 14 | 0.25 mg | 0/2 (0%) | — | |
| 2 | 0.5 mg | 3/3 (100%) | All >99% | |
| 900 | 14 | 0.25 mg | 6/6 (100%) | All >99% |
| 2 | 0.5 mg | 3/3 (100%) | All >99% | |
| Radiation (rad) . | Anti-CD154 mAb . | Number of Chimeric Mice (%) . | Percentage of Donor-Origin PBMCs in Chimeric Mice . | |
|---|---|---|---|---|
| Number of Injections . | Dose . | |||
| 600 | 0 | — | 0/6 (0%) | — |
| 14 | 0.25 mg | 3/6 (50%) | 24%, 93%, >99% | |
| 2 | 0.5 mg | 8/12 (67%) | 95%, 97%, Six >99% | |
| 700 | 0 | — | 4/4 (100%) | All >99% |
| 14 | 0.25 mg | 1/3 (33%) | All >99% | |
| 2 | 0.5 mg | 5/6 (83%) | All >99% | |
| 800 | 0 | — | 2/2 (100%) | Both >99% |
| 14 | 0.25 mg | 0/2 (0%) | — | |
| 2 | 0.5 mg | 3/3 (100%) | All >99% | |
| 900 | 14 | 0.25 mg | 6/6 (100%) | All >99% |
| 2 | 0.5 mg | 3/3 (100%) | All >99% | |
Spontaneously diabetic NOD/Lt mice (H2g7) were irradiated, transfused intravenously with 25 × 106C57BL/6 (H2b) bone marrow cells, and injected intraperitoneally with anti-CD154 mAb as indicated. The schedule of anti-CD154 mAb (relative to irradiation and bone marrow transplantation on day 0) was as follows. Recipients given 2 injections were treated on days 0 and +3, and recipients of 14 injections on days −3, 0, and twice weekly thereafter. The percentage of H2bdonor-origin PBMCs was determined by flow cytometry 4 to 6 weeks after irradiation and bone marrow transplantation. The presence of chimerism was defined as at least 1% donor-origin cells.