In vitro proliferative response
| Stimuli . | Thymus . | Spleen . | Lymph Node . |
|---|---|---|---|
| — | |||
| WT | 244 ± 20 | 16 062 ± 1117 | 2483 ± 196 |
| CD69 (−/−) | 141 ± 20 | 17 566 ± 892 | 1886 ± 349 |
| ConA | |||
| WT | 6635 ± 714 | 51 101 ± 1684 | 27 170 ± 2532 |
| CD69 (−/−) | 6181 ± 920 | 48 351 ± 699 | 25 802 ± 2532 |
| α-CD3 | |||
| WT | 5252 ± 1702 | 117 254 ± 15 912 | 71 121 ± 16 090 |
| CD69 (−/−) | 5516 ± 497 | 95 288 ± 19 426 | 54 115 ± 7342 |
| SEB | |||
| WT | 1007 ± 237 | 92 218 ± 4051 | 65 274 ± 5013 |
| CD69 (−/−) | 692 ± 84 | 110 317 ± 27 564 | 40 262 ± 5636 |
| LPS | |||
| WT | 3196 ± 116 | 61 186 ± 3783 | 118 482 ± 5326 |
| CD69 (−/−) | 1631 ± 203 | 70 372 ± 5650 | 79 867 ± 10 036 |
| Stimuli . | Thymus . | Spleen . | Lymph Node . |
|---|---|---|---|
| — | |||
| WT | 244 ± 20 | 16 062 ± 1117 | 2483 ± 196 |
| CD69 (−/−) | 141 ± 20 | 17 566 ± 892 | 1886 ± 349 |
| ConA | |||
| WT | 6635 ± 714 | 51 101 ± 1684 | 27 170 ± 2532 |
| CD69 (−/−) | 6181 ± 920 | 48 351 ± 699 | 25 802 ± 2532 |
| α-CD3 | |||
| WT | 5252 ± 1702 | 117 254 ± 15 912 | 71 121 ± 16 090 |
| CD69 (−/−) | 5516 ± 497 | 95 288 ± 19 426 | 54 115 ± 7342 |
| SEB | |||
| WT | 1007 ± 237 | 92 218 ± 4051 | 65 274 ± 5013 |
| CD69 (−/−) | 692 ± 84 | 110 317 ± 27 564 | 40 262 ± 5636 |
| LPS | |||
| WT | 3196 ± 116 | 61 186 ± 3783 | 118 482 ± 5326 |
| CD69 (−/−) | 1631 ± 203 | 70 372 ± 5650 | 79 867 ± 10 036 |
Mononuclear cells obtained from thymus, spleen, and lymph nodes from CD69 (−/−) mice and control littermates were stimulated in a proliferation assay. The results shown are the mean of triplicate cultures and are representative of six separate experiments. LPS = lipopolysaccharide; SEB = staphylococcus enterotoxin B; WT = wild type.