Effect of growth conditions, warfarin, and vitamin K on protein S messenger RNA expression in human vascular smooth muscle cells (HVSMCs)*
| . | Incubation (hours) . | Control . | Warfarin . | Vitamin K . |
|---|---|---|---|---|
| Growing HVSMCs | 24 48 | 3.6 3.4 | 3.7 3.3 | 3.9 3.7 |
| Growth-arrested HVSMCs | 24 48 | 3.2 3.3 | 3.6 3.6 | 3.4 3.5 |
| . | Incubation (hours) . | Control . | Warfarin . | Vitamin K . |
|---|---|---|---|---|
| Growing HVSMCs | 24 48 | 3.6 3.4 | 3.7 3.3 | 3.9 3.7 |
| Growth-arrested HVSMCs | 24 48 | 3.2 3.3 | 3.6 3.6 | 3.4 3.5 |
HVSMC cultures at 90% confluency were either placed in 10% fetal calf serum containing medium (growing) or 0.5% fetal calf serum (growth arrested) and were incubated with either 1 μg/mL warfarin, 1 μg/mL vitamin K, or vehicle control. Extracted RNA was subjected to Northern blotting using a protein S complementary DNA probe, followed by stripping and reprobing with a G6PD probe. Values represent ratios of protein S/G6PD bound probe as determined by densitometric scanning.