Primer sequences and PCR conditions for the evaluation of IFN mRNA expression
| Primer . | Nucleotide Sequence (5′-3′) . | Bp* . | No. Cycles . |
|---|---|---|---|
| β actin | 391 | 30 | |
| Sense | TGACGGGGTCACCCACACTGTGCCCATCTA | ||
| Antisense | CTAGAAGCATTGCGGTGGAGCATGGAGGG | ||
| Murine IFN-α1-2 | 162 | 40 | |
| Sense | TGTCTGATGCAGCAGGTGG | ||
| Antisense | AAGACAGGGCTCTCCAGAC | ||
| Murine IFN-β | 156 | 40 | |
| Sense | CCATCCAAGAGATGCTCCAG | ||
| Antisense | GTGGAGAGCAGTTGAGGACA |
| Primer . | Nucleotide Sequence (5′-3′) . | Bp* . | No. Cycles . |
|---|---|---|---|
| β actin | 391 | 30 | |
| Sense | TGACGGGGTCACCCACACTGTGCCCATCTA | ||
| Antisense | CTAGAAGCATTGCGGTGGAGCATGGAGGG | ||
| Murine IFN-α1-2 | 162 | 40 | |
| Sense | TGTCTGATGCAGCAGGTGG | ||
| Antisense | AAGACAGGGCTCTCCAGAC | ||
| Murine IFN-β | 156 | 40 | |
| Sense | CCATCCAAGAGATGCTCCAG | ||
| Antisense | GTGGAGAGCAGTTGAGGACA |
Number of base pairs of the amplified fragment.