PMNLs were incubated with 10 μmol/L ML-7 or 10 mmol/L BDM for 10 minutes at 37°C before stimulation. For Syk phosphorylation, PMNLs were challenged with EIgG for 3 minutes, lysed, and immunoblotted with anti-phosphotyrosine; blots were scanned with a densitometer. For measurement of Ca⁺⁺ transients, PMNLs were loaded with fluo-3 and stimulated with 100 nmol/L fMLP, and fluorescence was monitored continuously; peak and duration of fluorescence were evaluated. Binding of EIgG was evaluated after 30 minutes at 4°C by counting the number of PMNLs with bound erythrocytes; each PMNL bound many erythrocytes. Data are expressed as mean ± SEM.