Test donor-derived cells/competitor-derived cells (T/C) ratio at 4 and 20 weeks after transplantation
| Day of Gestation . | No. of Cells Tested . | T/C Ratio (mean ± SD) . | |
|---|---|---|---|
| 4 wk . | 20 wk . | ||
| 11 | 2.0 × 105 | nd | nd |
| 12 | 5.0 × 105 | 1.98 ± 1.48 (n = 9) | 7.18 ± 7.39 (n = 9) |
| 10.0 × 105 | 2.37 ± 1.49 (n = 8) | 11.53 ± 11.10 (n = 8) | |
| 15.0 × 105 | 4.79 ± 2.60 (n = 9) | 17.60 ± 7.08 (n = 9) | |
| 13 | 0.8 × 105 | 0.35 ± 0.20 (n = 5) | 0.86 ± 0.59 (n = 5) |
| 1.0 × 105 | 0.32 ± 0.25 (n = 5) | 1.36 ± 1.16 (n = 5) | |
| 2.0 × 105 | 0.77 ± 0.38 (n = 5) | 2.48 ± 1.51 (n = 5) | |
| 14 | 1.0 × 105 | 0.30 ± 0.18 (n = 5) | 1.44 ± 1.22 (n = 5) |
| 1.5 × 105 | 0.50 ± 0.10 (n = 5) | 1.60 ± 1.78 (n = 5) | |
| 2.0 × 105 | 0.64 ± 0.10 (n = 8) | 2.32 ± 1.05 (n = 8) | |
| 15 | 1.0 × 105 | 0.31 ± 0.30 (n = 9) | 1.20 ± 1.15 (n = 9) |
| 2.0 × 105 | 0.78 ± 0.42 (n = 7) | 2.95 ± 2.31 (n = 7) | |
| 3.0 × 105 | 1.10 ± 0.67 (n = 9) | 3.35 ± 1.95 (n = 9) | |
| 16 | 1.0 × 105 | 0.31 ± 0.19 (n = 5) | 1.40 ± 1.11 (n = 5) |
| 2.5 × 105 | 1.10 ± 0.75 (n = 5) | 3.29 ± 1.39 (n = 5) | |
| 5.0 × 105 | 1.53 ± 0.54 (n = 5) | 6.72 ± 2.78 (n = 5) | |
| 17 | 1.0 × 105 | 0.33 ± 0.16 (n = 8) | 1.15 ± 0.96 (n = 8) |
| 2.0 × 105 | 0.66 ± 0.47 (n = 6) | 2.13 ± 1.65 (n = 6) | |
| 3.0 × 105 | 1.63 ± 1.62 (n = 8) | 4.39 ± 2.94 (n = 8) | |
| 18 | 1.0 × 105 | 0.48 ± 0.28 (n = 5) | 1.31 ± 0.78 (n = 5) |
| 2.5 × 105 | 1.32 ± 0.65 (n = 5) | 2.39 ± 0.90 (n = 5) | |
| 5.0 × 105 | 2.63 ± 0.74 (n = 5) | 5.95 ± 1.93 (n = 5) | |
| Day of Gestation . | No. of Cells Tested . | T/C Ratio (mean ± SD) . | |
|---|---|---|---|
| 4 wk . | 20 wk . | ||
| 11 | 2.0 × 105 | nd | nd |
| 12 | 5.0 × 105 | 1.98 ± 1.48 (n = 9) | 7.18 ± 7.39 (n = 9) |
| 10.0 × 105 | 2.37 ± 1.49 (n = 8) | 11.53 ± 11.10 (n = 8) | |
| 15.0 × 105 | 4.79 ± 2.60 (n = 9) | 17.60 ± 7.08 (n = 9) | |
| 13 | 0.8 × 105 | 0.35 ± 0.20 (n = 5) | 0.86 ± 0.59 (n = 5) |
| 1.0 × 105 | 0.32 ± 0.25 (n = 5) | 1.36 ± 1.16 (n = 5) | |
| 2.0 × 105 | 0.77 ± 0.38 (n = 5) | 2.48 ± 1.51 (n = 5) | |
| 14 | 1.0 × 105 | 0.30 ± 0.18 (n = 5) | 1.44 ± 1.22 (n = 5) |
| 1.5 × 105 | 0.50 ± 0.10 (n = 5) | 1.60 ± 1.78 (n = 5) | |
| 2.0 × 105 | 0.64 ± 0.10 (n = 8) | 2.32 ± 1.05 (n = 8) | |
| 15 | 1.0 × 105 | 0.31 ± 0.30 (n = 9) | 1.20 ± 1.15 (n = 9) |
| 2.0 × 105 | 0.78 ± 0.42 (n = 7) | 2.95 ± 2.31 (n = 7) | |
| 3.0 × 105 | 1.10 ± 0.67 (n = 9) | 3.35 ± 1.95 (n = 9) | |
| 16 | 1.0 × 105 | 0.31 ± 0.19 (n = 5) | 1.40 ± 1.11 (n = 5) |
| 2.5 × 105 | 1.10 ± 0.75 (n = 5) | 3.29 ± 1.39 (n = 5) | |
| 5.0 × 105 | 1.53 ± 0.54 (n = 5) | 6.72 ± 2.78 (n = 5) | |
| 17 | 1.0 × 105 | 0.33 ± 0.16 (n = 8) | 1.15 ± 0.96 (n = 8) |
| 2.0 × 105 | 0.66 ± 0.47 (n = 6) | 2.13 ± 1.65 (n = 6) | |
| 3.0 × 105 | 1.63 ± 1.62 (n = 8) | 4.39 ± 2.94 (n = 8) | |
| 18 | 1.0 × 105 | 0.48 ± 0.28 (n = 5) | 1.31 ± 0.78 (n = 5) |
| 2.5 × 105 | 1.32 ± 0.65 (n = 5) | 2.39 ± 0.90 (n = 5) | |
| 5.0 × 105 | 2.63 ± 0.74 (n = 5) | 5.95 ± 1.93 (n = 5) | |
Competitive repopulation was performed with 3 different numbers of fetal liver cells for each day of gestation. A whole liver was transplanted for day 11 of gestation. At 4 and 20 weeks after transplantation, peripheral blood cells were analyzed to evaluate engraftment. T/C ratio, % test donor-derived cells divided by % competitor-derived cells on FACS analysis (see Figure 1). nd, not determined because of undetectable level of engraftment. The numbers of recipients analyzed are shown in parentheses.