HepG2 cells in monolayer were washed and incubated at 37°C for 4 h with media containing 0.6 nmol ¹²⁵I-TFPI in the presence or absence of the indicated competitors. Thereafter, the media was removed and assayed for ¹²⁵I-TFPI degradation products as described in the text. Each value represents the mean of triplicate determinations.

HepG2 cells in monolayer were washed and incubated at 4°C for 2 h with media containing 20 nmol ¹²⁵I-TFPI in the presence or absence of the indicated competitors. Thereafter, the media was removed, the cells rinsed and cell-surface bound ¹²⁵I-TFPI was determined as described in the text. Each value represents the mean of triplicate determinations.