Estrogen alters B lineage precursor populations in normal and mutant mice
| Mouse . | N . | CD19+CD43+ . | CD19+CD43− . |
|---|---|---|---|
| BALB/c ctr | 5 | 6.0 ± 1.4* | 36.5 ± 10.9 |
| BALB/c E2 | 5 | 5.7 ± 2.0 | 15.9 ± 3.5 |
| 3-83 μκ ctr | 2 | 2.2 ± 0.7 | 21.9 ± 1.5 |
| 3-83 μκ E2 | 2 | 1.0 ± 0.1 | 8.8 ± 0.1 |
| RAG-1−/− hu μ ctr | 1 | 1.2 | 30.9 |
| RAG-1−/−hu μ E2 | 1 | 0.2 | 7.4 |
| RAG-1−/− ctr | 2 | 21.1 ± 5.3 | 2.4 ± 0.7 |
| RAG-1−/− E2 | 2 | 10.7 ± 0.8 | 15.9 ± 0.1 |
| RAG-2−/− ctr | 3 | 12.6 ± 2.5 | 1.4 ± 0.3 |
| RAG-2−/−E2 | 3 | 7.0 ± 1.2 | 7.8 ± 2.3 |
| Mouse . | N . | CD19+CD43+ . | CD19+CD43− . |
|---|---|---|---|
| BALB/c ctr | 5 | 6.0 ± 1.4* | 36.5 ± 10.9 |
| BALB/c E2 | 5 | 5.7 ± 2.0 | 15.9 ± 3.5 |
| 3-83 μκ ctr | 2 | 2.2 ± 0.7 | 21.9 ± 1.5 |
| 3-83 μκ E2 | 2 | 1.0 ± 0.1 | 8.8 ± 0.1 |
| RAG-1−/− hu μ ctr | 1 | 1.2 | 30.9 |
| RAG-1−/−hu μ E2 | 1 | 0.2 | 7.4 |
| RAG-1−/− ctr | 2 | 21.1 ± 5.3 | 2.4 ± 0.7 |
| RAG-1−/− E2 | 2 | 10.7 ± 0.8 | 15.9 ± 0.1 |
| RAG-2−/− ctr | 3 | 12.6 ± 2.5 | 1.4 ± 0.3 |
| RAG-2−/−E2 | 3 | 7.0 ± 1.2 | 7.8 ± 2.3 |
Data reflect percentages ± SD of bone marrow cells within lymphocyte light scatter gates representing B-lineage precursor populations. The CD19+CD43− population in both BALB/c and 3-83 μκ mice is inclusive of sIgM+cells. N denotes the number of animals analyzed. The RAG-1−/− mice in this table were on the same background strain as the RAG-1−/− hu μ transgenic. Similar results were obtained with RAG-1−/−(M) and RAG-1−/−(S) mice (Figure 8 and data not shown).