Effects of SCM-LIF on the maintenance and expansion of freshly purified human fetal BM CD34+thy-1+ cells in vitro
| Culture Conditions . | Frequency of CD34+ thy-1+–Positive Wells . | Percentage of CD34+ thy-1+Cells . |
|---|---|---|
| Positive control | 100% (10/10) | 8 ± 2 |
| Negative control | 0% (0/10) | N/A |
| 0% SCM-LIF | 0% (0/10) | N/A |
| 5% SCM-LIF | 0% (0/10) | N/A |
| 10% SCM-LIF | 10% (1/10) | 3.6 |
| 25% SCM-LIF | 40% (4/10) | 5 ± 3 |
| 50% SCM-LIF | 70% (7/10) | 10 ± 4 |
| 100% SCM-LIF | 100% (10/10) | 14 ± 4 |
| 200% SCM-LIF | 100% (10/10) | 18 ± 4 |
| 400% SCM-LIF | 100% (10/10) | 18 ± 3 |
| Culture Conditions . | Frequency of CD34+ thy-1+–Positive Wells . | Percentage of CD34+ thy-1+Cells . |
|---|---|---|
| Positive control | 100% (10/10) | 8 ± 2 |
| Negative control | 0% (0/10) | N/A |
| 0% SCM-LIF | 0% (0/10) | N/A |
| 5% SCM-LIF | 0% (0/10) | N/A |
| 10% SCM-LIF | 10% (1/10) | 3.6 |
| 25% SCM-LIF | 40% (4/10) | 5 ± 3 |
| 50% SCM-LIF | 70% (7/10) | 10 ± 4 |
| 100% SCM-LIF | 100% (10/10) | 14 ± 4 |
| 200% SCM-LIF | 100% (10/10) | 18 ± 4 |
| 400% SCM-LIF | 100% (10/10) | 18 ± 3 |
A well is scored as CD34+ thy-1+–positive only if it has detectable (>1%) CD34+ thy-1+cells in the well. Numbers in parentheses indicate the total number of CD34+ thy-1+–positive wells divided by the total number of wells analyzed in the experiments. Data for the percentage of CD34+ thy-1+ cells in the CD34+ thy-1+–positive wells are presented as the mean ± SD of the total number of CD34+thy-1+–positive wells in each culture condition. N/A means not applicable.