Relative expression of CD164 epitopes on CD34 and AC133 subsets of human fetal liver cells
| Cell Subset . | % CD34 Cells Expressing CD164 and AC133 Epitopes (mean ± SD; n = 3) . | Median Fluorescence Intensity of CD164 Labeling for Each Specified CD34+AC133 Subset (mean ± SD; n = 3) . | ||||
|---|---|---|---|---|---|---|
| Class I 105A5 . | Class II 103B2/9E10 . | Class III N6B6 . | Class I 105A5 . | Class II 103B2/9E10 . | Class III N6B6 . | |
| CD34+AC133+CD164+ | 58 ± 14 | 79 ± 4 | 79 ± 5 | 40 ± 12 | 259 ± 128 | 205 ± 104 |
| CD34+AC133loCD164+ | 12 ± 8 | 15 ± 7 | 17 ± 7 | 36 ± 9 | 133 ± 11 | 99 ± 31 |
| CD34+AC133+CD164lo | 21 ± 12 | 2 ± 0 | 1 ± 0 | 11 ± 1 | 11 ± 0 | 12 ± 1 |
| CD34+AC133loCD164lo | 9 ± 2 | 5 ± 3 | 3 ± 2 | 7 ± 1 | 6 ± 0 | 10 ± 1 |
| Cell Subset . | % CD34 Cells Expressing CD164 and AC133 Epitopes (mean ± SD; n = 3) . | Median Fluorescence Intensity of CD164 Labeling for Each Specified CD34+AC133 Subset (mean ± SD; n = 3) . | ||||
|---|---|---|---|---|---|---|
| Class I 105A5 . | Class II 103B2/9E10 . | Class III N6B6 . | Class I 105A5 . | Class II 103B2/9E10 . | Class III N6B6 . | |
| CD34+AC133+CD164+ | 58 ± 14 | 79 ± 4 | 79 ± 5 | 40 ± 12 | 259 ± 128 | 205 ± 104 |
| CD34+AC133loCD164+ | 12 ± 8 | 15 ± 7 | 17 ± 7 | 36 ± 9 | 133 ± 11 | 99 ± 31 |
| CD34+AC133+CD164lo | 21 ± 12 | 2 ± 0 | 1 ± 0 | 11 ± 1 | 11 ± 0 | 12 ± 1 |
| CD34+AC133loCD164lo | 9 ± 2 | 5 ± 3 | 3 ± 2 | 7 ± 1 | 6 ± 0 | 10 ± 1 |
Purified CD34+ cells from fetal liver cells were labeled with CD34-PerCP, AC133-PE, and the different CD164 MAbs plus FITC-conjugated anti-isotype secondary antibodies as described in “Materials and methods.” The percentages of cells falling within each quadrant were determined by comparison with the negative isotype-matched control MAbs. Values represent means ± SD of 3 independent experiments.