Functional characteristics and molecular profile of fresh isolated CD34+ and functionally isolated CLRPP
| . | CD34+ . | CLRPP . |
|---|---|---|
| CD34% | 96 | 99 |
| CFU-GM/1000 cells | 60 | 270 |
| BFU-E/1000 cells | 40 | 240 |
| LTC-IC frequency | 1:400 | 1:100 |
| SG2M% | 3 | 30 |
| GATA-1 RNA (relative adsorbance)* | 0.912 | 0.651 |
| PU.1 RNA (relative adsorbance)* | 0.767 | 0.674 |
| bcl-2 protein (relative adsorbance)† | 0.425 | 0.967 |
| bcl-xL protein (relative adsorbance)† | 0.122 | 0.562 |
| . | CD34+ . | CLRPP . |
|---|---|---|
| CD34% | 96 | 99 |
| CFU-GM/1000 cells | 60 | 270 |
| BFU-E/1000 cells | 40 | 240 |
| LTC-IC frequency | 1:400 | 1:100 |
| SG2M% | 3 | 30 |
| GATA-1 RNA (relative adsorbance)* | 0.912 | 0.651 |
| PU.1 RNA (relative adsorbance)* | 0.767 | 0.674 |
| bcl-2 protein (relative adsorbance)† | 0.425 | 0.967 |
| bcl-xL protein (relative adsorbance)† | 0.122 | 0.562 |
Results are expressed as the average values observed in 6 different isolation experiments established with cells collected from 6 different patients.
Expressed as the relative adsorbance ratios between the RNA levels of the gene of interest and that of β-actin.
Expressed as the relative adsorbance ratios between the protein levels of the molecule of interest and that of actin at Western blot analysis.