Detection of TCR-γ and immunoglobulin gene rearrangements in single Hodgkin and Reed-Sternberg cells of 19 cases of classic HD
| Case . | Age/Sex . | Phenotype . | TCR-γ . | Immunoglobulin Heavy-Chain Gene . | Immunoglobulin Light-Chain Gene . | |||
|---|---|---|---|---|---|---|---|---|
| Isolated Cells . | Rearrangements (I/N) . | Isolated Cells . | Rearrangements (I/N) . | Isolated Cells . | Rearrangements (I/N) . | |||
| 1 | 56/M | T | 35 | 12/0 | 19 | 0/0 | 15 | 0/0 |
| 2 | 72/M | T | 30 | 9/0 | 16 | 0/0 | 12 | 0/0 |
| 3 | 27/M | T | 25 | 0/0 | 12 | 0/0 | 20 | 8/0 |
| 4 | 34/M | T | 20 | 0/0 | 27 | 10/0 | 12 | 0/0 |
| 5 | Na | T | 24 | 0/0 | 21 | 6/0 | nd | |
| 6 | Na | T | 14 | 0/0 | 12 | 3/0 | nd | |
| 7 | 31/F | T | 12 | 0/0 | 34 | 3/0 | nd | |
| 8 | 12/F | T | 12 | 0/0 | 10 | 0/0 | 23 | 4/0 |
| 9 | 41/F | T | 13 | 0/0 | nd | nd | ||
| 10 | 24/M | T | 29 | 0/0 | nd | nd | ||
| 11 | 27/F | T | 12 | 0/0 | nd | nd | ||
| 12 | 23/M | T | 14 | 0/0 | nd | nd | ||
| 13 | 23/M | T | 12 | 0/0 | nd | nd | ||
| 14 | 32/M | 0 | 12 | 0/0 | 23 | 9/0 | 14 | 5/0 |
| 15 | 80/F | 0 | 12 | 0/0 | 16 | 6/0 | 13 | 4/0 |
| 16 | 35/M | 0 | 12 | 0/0 | 28 | 10/0 | 12 | 6/0 |
| 17 | 38/F | 0 | 12 | 0/0 | 18 | 6/0 | 18 | 0/0 |
| 18 | 30/M | 0 | 12 | 0/0 | 16 | 6/0 | 20 | 0/0 |
| 19 | 38/M | 0 | 29 | 0/0 | 25 | 0/0 | 14 | 5/0 |
| Case . | Age/Sex . | Phenotype . | TCR-γ . | Immunoglobulin Heavy-Chain Gene . | Immunoglobulin Light-Chain Gene . | |||
|---|---|---|---|---|---|---|---|---|
| Isolated Cells . | Rearrangements (I/N) . | Isolated Cells . | Rearrangements (I/N) . | Isolated Cells . | Rearrangements (I/N) . | |||
| 1 | 56/M | T | 35 | 12/0 | 19 | 0/0 | 15 | 0/0 |
| 2 | 72/M | T | 30 | 9/0 | 16 | 0/0 | 12 | 0/0 |
| 3 | 27/M | T | 25 | 0/0 | 12 | 0/0 | 20 | 8/0 |
| 4 | 34/M | T | 20 | 0/0 | 27 | 10/0 | 12 | 0/0 |
| 5 | Na | T | 24 | 0/0 | 21 | 6/0 | nd | |
| 6 | Na | T | 14 | 0/0 | 12 | 3/0 | nd | |
| 7 | 31/F | T | 12 | 0/0 | 34 | 3/0 | nd | |
| 8 | 12/F | T | 12 | 0/0 | 10 | 0/0 | 23 | 4/0 |
| 9 | 41/F | T | 13 | 0/0 | nd | nd | ||
| 10 | 24/M | T | 29 | 0/0 | nd | nd | ||
| 11 | 27/F | T | 12 | 0/0 | nd | nd | ||
| 12 | 23/M | T | 14 | 0/0 | nd | nd | ||
| 13 | 23/M | T | 12 | 0/0 | nd | nd | ||
| 14 | 32/M | 0 | 12 | 0/0 | 23 | 9/0 | 14 | 5/0 |
| 15 | 80/F | 0 | 12 | 0/0 | 16 | 6/0 | 13 | 4/0 |
| 16 | 35/M | 0 | 12 | 0/0 | 28 | 10/0 | 12 | 6/0 |
| 17 | 38/F | 0 | 12 | 0/0 | 18 | 6/0 | 18 | 0/0 |
| 18 | 30/M | 0 | 12 | 0/0 | 16 | 6/0 | 20 | 0/0 |
| 19 | 38/M | 0 | 29 | 0/0 | 25 | 0/0 | 14 | 5/0 |
Cases 1-13: Expression of T-cell antigens. Cases 14-19: Absence of B-cell and T-cell antigens. I, indicates identical rearrangements; N, nonidentical rearrangements; F, female; M, male; na, not available; nd, not done.