Phenotype of transformed macaque T cells and infusion conditions
| Animal . | Phenotype of transformed T cells . | Infusion conditions . | |||||
|---|---|---|---|---|---|---|---|
| CD4+ CD8− . | CD4− CD8+ . | CD4+ CD8+ . | Generation of T cell lines . | Positive results out of attempted virus isolations . | Body weight (kg) . | Number of infused T cells × 106 . | |
| Mm5574 | − | + | − | Polyclonal culture | 5/5 | 7.5 | 600 |
| Mm7067 | + | − | + | Limiting dilution | 4/6 | 4.0 | 320 |
| Mf6311 | − | + | − | Limiting dilution | 0/4 | 2.1 | 170 |
| Mf6490 | + | − | + | Limiting dilution | 1/4 | 2.5 | 200 |
| Mf6698 | − | − | + | Limiting dilution | 3/4 | 1.6 | 130 |
| Animal . | Phenotype of transformed T cells . | Infusion conditions . | |||||
|---|---|---|---|---|---|---|---|
| CD4+ CD8− . | CD4− CD8+ . | CD4+ CD8+ . | Generation of T cell lines . | Positive results out of attempted virus isolations . | Body weight (kg) . | Number of infused T cells × 106 . | |
| Mm5574 | − | + | − | Polyclonal culture | 5/5 | 7.5 | 600 |
| Mm7067 | + | − | + | Limiting dilution | 4/6 | 4.0 | 320 |
| Mf6311 | − | + | − | Limiting dilution | 0/4 | 2.1 | 170 |
| Mf6490 | + | − | + | Limiting dilution | 1/4 | 2.5 | 200 |
| Mf6698 | − | − | + | Limiting dilution | 3/4 | 1.6 | 130 |
CD4 and CD8 are frequently coexpressed on cultivated parental and transformed macaque T cells.10 The virus production was analyzed with enhanced sensitivity from supernatants of growth-transformed T-cell cultures.