Stabilization of HLA-A2 molecules from acid-stripped platelets after addition of exogenous HLA-A2 binding peptide and β2-microglobulin
| . | Neg. Control . | W6/32 . | BB7.2 . |
|---|---|---|---|
| Time 0 | 43-150 | 212 | 109 |
| Stripping buffer treatment3-151 | |||
| Medium | 10 | 87 | 31 |
| β2m | 9 | 116 | 28 |
| β2m + Ras 8-163-152 | 9 | 56 | 22 |
| β2m + Mat 58-66 | 20 | 186 | 80 |
| β2m + gp190 367-375 | 9 | 179 | 45 |
| . | Neg. Control . | W6/32 . | BB7.2 . |
|---|---|---|---|
| Time 0 | 43-150 | 212 | 109 |
| Stripping buffer treatment3-151 | |||
| Medium | 10 | 87 | 31 |
| β2m | 9 | 116 | 28 |
| β2m + Ras 8-163-152 | 9 | 56 | 22 |
| β2m + Mat 58-66 | 20 | 186 | 80 |
| β2m + gp190 367-375 | 9 | 179 | 45 |
Staining with MoAbs is expressed as fluorescence mean. Stabilizations induced by HLA-A2-binding peptides are indicated in bold.
Platelets were treated for 30 seconds with stripping buffer. After 2 washes, peptides (100 μg/mL) and β2-microglobulin (β2m) (3 μg/mL) were added and platelets were further incubated for 2 hours at 37°C before staining. Detailed protocol is described in Materials and methods.
Ras peptide, an HLA-A11 binder, was used as negative control.