Phenotype and EGFP expression in human cord blood cells used for transplantation into NOD/SCID mice
| Cord Blood Ref . | HGFs . | % CD34+ . | Fold Expansion (post vs preinfection) . | % EGFP+ in: . | ||||
|---|---|---|---|---|---|---|---|---|
| Preinfection . | Postinfection . | Total Cells . | CD34+ . | Total Cells . | CD34+ Cells . | CD34+/ CD38low Cells . | ||
| CB-4 | M/S/F/6/3 | 92.3 | 58.7 | 5.8x | 3.7x | 63.7 | 58.8 | 63.6 |
| CB-5 | M/S/F/6/3 | 92.1 | 88.1 | 0.8x | 0.8x | 72.1 | 71.2 | 35.6 |
| CB-9 | M/S/F/6/3 | 80.5 | 69.3 | 5.0x | 4.4x | 90.8 | 93.6 | 81.6 |
| CB-10 | M/S/F/6/3 | 94.0 | 74.5 | 3.4x | 2.7x | 92.1 | 93.3 | 86.7 |
| CB-14 | M/S/F/6/3 | 97.1 | 48.2 | 2.5x | 1.3x | 60.7 | 64.0 | 53.7 |
| CB-15 | M/S/F/6/3 | 94.2 | 46.7 | 2.2x | 1.1x | 68.4 | 77.2 | 59.4 |
| CB-16 | M/S/F | 79.6 | 64.2 | 1.0x | 0.9x | 32.0 | 37.8 | 34.5 |
| CB-17 | M/S/F | 86.4 | 65.1 | 2.1x | 1.6x | 30.5 | 33.3 | 30.4 |
| Mean ± SE | 89.5 ± 2.3 | 64.4 ± 4.8 | 2.8 ± 0.6 | 2.0 ± 0.5 | 63.8 ± 8.2 | 66.2 ± 8.0 | 55.7 ± 7.6 | |
| Cord Blood Ref . | HGFs . | % CD34+ . | Fold Expansion (post vs preinfection) . | % EGFP+ in: . | ||||
|---|---|---|---|---|---|---|---|---|
| Preinfection . | Postinfection . | Total Cells . | CD34+ . | Total Cells . | CD34+ Cells . | CD34+/ CD38low Cells . | ||
| CB-4 | M/S/F/6/3 | 92.3 | 58.7 | 5.8x | 3.7x | 63.7 | 58.8 | 63.6 |
| CB-5 | M/S/F/6/3 | 92.1 | 88.1 | 0.8x | 0.8x | 72.1 | 71.2 | 35.6 |
| CB-9 | M/S/F/6/3 | 80.5 | 69.3 | 5.0x | 4.4x | 90.8 | 93.6 | 81.6 |
| CB-10 | M/S/F/6/3 | 94.0 | 74.5 | 3.4x | 2.7x | 92.1 | 93.3 | 86.7 |
| CB-14 | M/S/F/6/3 | 97.1 | 48.2 | 2.5x | 1.3x | 60.7 | 64.0 | 53.7 |
| CB-15 | M/S/F/6/3 | 94.2 | 46.7 | 2.2x | 1.1x | 68.4 | 77.2 | 59.4 |
| CB-16 | M/S/F | 79.6 | 64.2 | 1.0x | 0.9x | 32.0 | 37.8 | 34.5 |
| CB-17 | M/S/F | 86.4 | 65.1 | 2.1x | 1.6x | 30.5 | 33.3 | 30.4 |
| Mean ± SE | 89.5 ± 2.3 | 64.4 ± 4.8 | 2.8 ± 0.6 | 2.0 ± 0.5 | 63.8 ± 8.2 | 66.2 ± 8.0 | 55.7 ± 7.6 | |
CD34+ cord blood cells were transduced as indicated in Materials and methods, and analyzed at the end of the last infection cycle before transplantation into NOD/SCID mice (NOD/SCID data are shown in Table 2).
HGFs indicates hematopoietic growth factors (3, IL-3; 6, IL-6; F, Flt3-ligand; M, megacaryocyte growth and development factor; S, stem cell factor).