GST-hDll1NDSL promotes the expansion of primitive hematopoietic progenitor cells neutralized by anti-mDll1NDSL antibodies
| Protein Groups . | Live Cells . | HPP-CFU* . | CFU-GM* . | CFU-Sday12* . |
|---|---|---|---|---|
| 1. GST | 6 (±2) | 1220 (±200) | 4503 (±491) | 22 (±13) |
| 2. GST-hDll1NDSL | 5 (±2) | 3011 (±547) | 7187 (±840) | 88 (±22) |
| 3. GST-hDll1NDSL Anti-mDll1 Ab | 5 (±2) | 1787 (±110 ) | 4277 (±327) | 34 (±25 ) |
| 4. GST-hDll1NDSL Preimmune serum | 6 (±2 ) | 3107 (±639) | 9657 (±770) | 72 (±19) |
| Protein Groups . | Live Cells . | HPP-CFU* . | CFU-GM* . | CFU-Sday12* . |
|---|---|---|---|---|
| 1. GST | 6 (±2) | 1220 (±200) | 4503 (±491) | 22 (±13) |
| 2. GST-hDll1NDSL | 5 (±2) | 3011 (±547) | 7187 (±840) | 88 (±22) |
| 3. GST-hDll1NDSL Anti-mDll1 Ab | 5 (±2) | 1787 (±110 ) | 4277 (±327) | 34 (±25 ) |
| 4. GST-hDll1NDSL Preimmune serum | 6 (±2 ) | 3107 (±639) | 9657 (±770) | 72 (±19) |
Lin− cells were isolated from 5-Fu-treated mouse bone marrow and cultured for 12 days in serum-free medium with IL-11, KL, and FL. GST, GST-hDll1NDSL, GST-hDll1NDSLplus anti-mDll1NDSL antibody, or GST-hDll1NDSLplus preimmune serum was added to the cultures every 3 days. Cells were evaluated for their cellularity, HPP-CFU (colonies larger than 0.5 mm in diameter), CFU-GM, and CFU-Sday12. Data are expressed as mean number of 3 independent experiments with SE in parentheses. Statistical significance is determined by the two-tailed paired Studentt-test. Differences between protein groups 1 and 3 and groups 2 and 4 are not statistically significant (P > .05 for all the observed parameters). Differences between protein groups 1 and 2 and protein groups 3 and 4 are statistically very significant for the parameters of HPP-CFU, CFU-GM, CFU-Sday12(*P < .01) but not significant for the live cells (P > .05).