hDll1NDSL functions similarly to GST-hDll1NDSL fusion protein
| Protein Groups . | Live Cells* . | Total CFU3-151 . | Differentiation . | Cell Cycle . | Apoptosis . | ||
|---|---|---|---|---|---|---|---|
| Mac-13-151 . | Gr-13-151 . | G0/G13-151 . | S3-151 . | Sub-G13-151 . | |||
| 1. GST | 263 (±40) | 3518 (±456) | 82 (±1) | 70 (±6) | 88 (±1) | 6 (±1) | 46 (±4) |
| 2. GST-hDll1NDSL | 300 (±76) | 15135 (±272) | 75 (±1) | 56 (±3) | 65 (±2) | 30 (±2) | 12 (±4) |
| 3. GST, Thrombin | 288 (±64) | 3240 (±216) | 87 (±4) | 81 (±3) | 89 (±1) | 6 (±1) | 46 (±10) |
| 4. hDll1NDSL, GST, Thrombin | 356 (±72) | 15766 (±992) | 75 (±4) | 61 (±4) | 70 (±3) | 25 (±4) | 16 (±3) |
| Protein Groups . | Live Cells* . | Total CFU3-151 . | Differentiation . | Cell Cycle . | Apoptosis . | ||
|---|---|---|---|---|---|---|---|
| Mac-13-151 . | Gr-13-151 . | G0/G13-151 . | S3-151 . | Sub-G13-151 . | |||
| 1. GST | 263 (±40) | 3518 (±456) | 82 (±1) | 70 (±6) | 88 (±1) | 6 (±1) | 46 (±4) |
| 2. GST-hDll1NDSL | 300 (±76) | 15135 (±272) | 75 (±1) | 56 (±3) | 65 (±2) | 30 (±2) | 12 (±4) |
| 3. GST, Thrombin | 288 (±64) | 3240 (±216) | 87 (±4) | 81 (±3) | 89 (±1) | 6 (±1) | 46 (±10) |
| 4. hDll1NDSL, GST, Thrombin | 356 (±72) | 15766 (±992) | 75 (±4) | 61 (±4) | 70 (±3) | 25 (±4) | 16 (±3) |
Mouse Lin− bone marrow cells were cultured for 7 days in serum-free medium with IL-3, GM-CSF, and IL-1. GST, GST-hDll1NDSL, GST plus thrombin, or hDll1NDSLplus GST plus thrombin were added to the cultures every 3 days. Cultured cells were evaluated for their cellularity, CFU, Mac-1/Gr-1 expression, and cell-cycle kinetics. Data are expressed as mean number of live cells and CFU from 3 separate experiments ± SE. Data for differentiation and cell-cycle analyses are expressed as mean percentage of 3 independent experiments with SD in parentheses. Statistical significance is determined by the two-tailed paired Studentt-test. Differences between protein groups 1 and 3 and groups 2 and 4 are not statistically significant (P > .05 for all the observed parameters). Differences between protein groups 1 and 2 and protein groups 3 and 4 are statistically significant for the parameters of total CFU, differentiation, cell cycle, and apoptosis (
P < .01), and significant for live cells (*P < .05).