GST has no effects in the culture of Lin−cells
| N = 3 . | Cytokines . | Cytokines + BSA (0.4 μmol/L) . | Cytokines + GST . | P Value . | |||
|---|---|---|---|---|---|---|---|
| 0.1 μmol/L . | 0.2 μmol/L . | 0.3 μmol/L . | 0.4 μmol/L . | ||||
| Live cells | 122 (±20) | 133 (±7) | 111 (±16) | 118 (±3) | 121 (±10) | 125 (±4) | >.05 |
| Total CFU | 918 (±122) | 732 (±9.1) | 808 (±88) | 767 (±59) | 908 (±57) | 846 (±42) | >.05 |
| Mac-1 cells (%) | 82 (±4.5) | 83 (±3.5) | 80 (±4) | 78 (±2.8) | 81 (±3.3) | 75 (±2.9) | >.05 |
| S-phase cells (%) | 26.8 (±2) | 29.3 (±3.1) | 25.1 (±2) | 25.5 (±4) | 23 (±3) | 23.3 (±1.7) | >.05 |
| N = 3 . | Cytokines . | Cytokines + BSA (0.4 μmol/L) . | Cytokines + GST . | P Value . | |||
|---|---|---|---|---|---|---|---|
| 0.1 μmol/L . | 0.2 μmol/L . | 0.3 μmol/L . | 0.4 μmol/L . | ||||
| Live cells | 122 (±20) | 133 (±7) | 111 (±16) | 118 (±3) | 121 (±10) | 125 (±4) | >.05 |
| Total CFU | 918 (±122) | 732 (±9.1) | 808 (±88) | 767 (±59) | 908 (±57) | 846 (±42) | >.05 |
| Mac-1 cells (%) | 82 (±4.5) | 83 (±3.5) | 80 (±4) | 78 (±2.8) | 81 (±3.3) | 75 (±2.9) | >.05 |
| S-phase cells (%) | 26.8 (±2) | 29.3 (±3.1) | 25.1 (±2) | 25.5 (±4) | 23 (±3) | 23.3 (±1.7) | >.05 |
Mouse Lin− bone marrow cells were cultured for 7 days in the serum-free medium supplemented with cytokines (IL-3, IL-1, and GM-CSF). The effects of 20 μg/mL bovine serum albumin and GST at different concentrations were tested under these culture conditions. Cultures were evaluated for their cellularity, total CFU, expression of Mac-1, and S-phase cells. Data are expressed as mean percentage of 3 independent experiments with SD in parentheses. Differences of the observed parameters among 6 tested groups are all not statistically significant as determined by overall F test for one-way analysis of variance (P > .05).