Sequences of PCR primers used for subcloning
| Primer . | Patient . | Ig Chain . | 5′-3′ Sequence . |
|---|---|---|---|
| CEP.1Hfor | 1 | Fd | ATCTGGCCGGCAAGGCCACCATGcaggtgcagctacagcagtg |
| CEP.2Hfor | 2 | Fd | ATCTGGCCGGCAAGGCCACCATGgagctgcagctggtggag |
| CEP.Hrev | 1, 2 | Fd | AGCTGGATCCATTATGCTCAATGATGATGATGATGATGgcaagatttgggctcaactttc |
| CEP.1Lfor | 1 | IgL | AGTCAGATCTGGCCGGCAAGGCCACCATGgacatccagatgacccagtctc |
| CEP.2Lfor | 2 | IgL | ATCTGGCCGGCAAGGCCACCATGgaaattgtattgagacagtctccag |
| CEP.Lrev | 1, 2 | IgL | AGCTGGATCCATTATGCTCAATGATGATGATGATGATGacactctccgcggttgaag |
| Primer . | Patient . | Ig Chain . | 5′-3′ Sequence . |
|---|---|---|---|
| CEP.1Hfor | 1 | Fd | ATCTGGCCGGCAAGGCCACCATGcaggtgcagctacagcagtg |
| CEP.2Hfor | 2 | Fd | ATCTGGCCGGCAAGGCCACCATGgagctgcagctggtggag |
| CEP.Hrev | 1, 2 | Fd | AGCTGGATCCATTATGCTCAATGATGATGATGATGATGgcaagatttgggctcaactttc |
| CEP.1Lfor | 1 | IgL | AGTCAGATCTGGCCGGCAAGGCCACCATGgacatccagatgacccagtctc |
| CEP.2Lfor | 2 | IgL | ATCTGGCCGGCAAGGCCACCATGgaaattgtattgagacagtctccag |
| CEP.Lrev | 1, 2 | IgL | AGCTGGATCCATTATGCTCAATGATGATGATGATGATGacactctccgcggttgaag |
SfiI and BamHI restriction sites are underlined. Kozak consensus sequences, translation start codons, and stop codons are depicted in bold. Regions annealing to the immunoglobulin cDNA are in small letters. CEP.1Lfor contains an additional BglII site immediately upstream of the SfiI site. CEP.Hrev and CEP.Lrev contain six histidine codons immediately upstream of the first stop codon.