Factor V activity of activated platelets
| Agonist . | Factor V Activity (mU/mL) . |
|---|---|
| None | 3.4 ± 0.2 |
| Convulxin (500 ng/mL) | 25.6 ± 3.7 |
| Thrombin (5 nmol/L) | 91.9 ± 12.9 |
| TRAP (100 μmol/L) | 17.3 ± 1.6 |
| A23187 (2 μmol/L) | 12.9 ± 1.5 |
| VCP (2 nmol/L) | 8.3 ± 1.7 |
| Thrombin + convulxin | 156.3 ± 18.9 |
| Thrombin + A23187 | 107.7 ± 24.0 |
| Convulxin + A23187 | 16.9 ± 1.1 |
| TRAP + VCP | 48.3 ± 10.4 |
| A23187 + VCP | 55.6 ± 8.5 |
| Convulxin + VCP | 67.7 ± 11.6 |
| Agonist . | Factor V Activity (mU/mL) . |
|---|---|
| None | 3.4 ± 0.2 |
| Convulxin (500 ng/mL) | 25.6 ± 3.7 |
| Thrombin (5 nmol/L) | 91.9 ± 12.9 |
| TRAP (100 μmol/L) | 17.3 ± 1.6 |
| A23187 (2 μmol/L) | 12.9 ± 1.5 |
| VCP (2 nmol/L) | 8.3 ± 1.7 |
| Thrombin + convulxin | 156.3 ± 18.9 |
| Thrombin + A23187 | 107.7 ± 24.0 |
| Convulxin + A23187 | 16.9 ± 1.1 |
| TRAP + VCP | 48.3 ± 10.4 |
| A23187 + VCP | 55.6 ± 8.5 |
| Convulxin + VCP | 67.7 ± 11.6 |
Platelets activated with various agonists were added to FV-deficient plasma and 1-stage clotting times were measured as described in “Materials and methods.” Clotting times were converted to FV activity (mU/mL) by comparison with a normal plasma. TRAP, thrombin receptor agonist peptide; VCP, Factor V coagulant protein. Values are expressed as mean ± SE; n = 3-8.