Effect of human CD40L on expressions of Fas, B7-1 and B7-2
| . | Fas . | B7-1 . | B7-2 . |
|---|---|---|---|
| NIH3T3/vt | 45% (+)* | 5% (−) | 23% (+) |
| NIH3T3/CD40LT | 94% (++) | 39% (+) | 74 (++) |
| . | Fas . | B7-1 . | B7-2 . |
|---|---|---|---|
| NIH3T3/vt | 45% (+)* | 5% (−) | 23% (+) |
| NIH3T3/CD40LT | 94% (++) | 39% (+) | 74 (++) |
A20 cells were cultured with NIH3T3/vt (control) and NIH3T3/CD40LT (CD40L) cells (100 A20 cells/1 NIH3T3 cell) for 48 hours in 10% FBS + RPMI1640. Cells were stained with FITC conjugated antibodies against mouse Fas, B7-1, and B7-2 molecules. Antibody-coated cells were enumerated by flow cytometric analysis.
Isotype control antibody staining were adjusted to be less than 5%, and percentage of positive cells were calculated. Intensity was judged according to previous report.9