Table 3.

Molecular characteristics of the transfectomas secreting AIDS-related lymphoma (ARL) immunoglobulin (Ig)

CaseIgH IgL
ARL-3  VH7.4.1/IgM  Vκ1-A20 
ARL-43-150 VH3-15/IgM  Vκ4  
ARL-7 VH3-15/IgM  Vκ1-26  
ARL-83-151 VH1-8/IgM  pSV2-GAS (κ)  
ARL-10 VH3-13/IgM  Vκ3-41  
ARL-14 VH4-34/IgM  Vκ4  
Negative control pRTM-GAS/IgM  pSV2-GAS (κ) 
CaseIgH IgL
ARL-3  VH7.4.1/IgM  Vκ1-A20 
ARL-43-150 VH3-15/IgM  Vκ4  
ARL-7 VH3-15/IgM  Vκ1-26  
ARL-83-151 VH1-8/IgM  pSV2-GAS (κ)  
ARL-10 VH3-13/IgM  Vκ3-41  
ARL-14 VH4-34/IgM  Vκ4  
Negative control pRTM-GAS/IgM  pSV2-GAS (κ) 
F3-150

Because the IgG chain gene could not be ligated into pcDNA vector, it has been ligated into pRTM vector and expressed as IgM.

F3-151

Because the IgL gene could not be cloned, a vector containing a germline-encoded V-kappa III gene was used for transfection.

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