Anti–human immunodeficiency virus enzyme-linked immunosorbent assay
| Antibodies | 450 OD |
| Negative controls* | Range 0.001 to 0.013 |
| Positive controls* | Range 0.546 to 1.637 |
| Cutoff† | 0.107 |
| 257-D-IV antibody‡ | 0.216 |
| ARL-3 | 0.010 |
| ARL-4 | 0.009 |
| ARL-7 | 0.009 |
| ARL-8 | 0.011 |
| ARL-10 | 0.009 |
| ARL-14 | 0.017 |
| Antibodies | 450 OD |
| Negative controls* | Range 0.001 to 0.013 |
| Positive controls* | Range 0.546 to 1.637 |
| Cutoff† | 0.107 |
| 257-D-IV antibody‡ | 0.216 |
| ARL-3 | 0.010 |
| ARL-4 | 0.009 |
| ARL-7 | 0.009 |
| ARL-8 | 0.011 |
| ARL-10 | 0.009 |
| ARL-14 | 0.017 |
ARL indicates AIDS-related lymphoma.
Controls provided by the kit (Abbott HIV AB HIV-1/HIV-2 3.0).
The cutoff value was determined by calculating the mean O.D. of three negative control experiments and adding 0.100 to the mean value.
Human anti-HIV-1 gp120 (IgG1) (NIH-AIDS Research and Reference Reagent Program Catalog).
Catalog #1510 was used as our positive control at 7 μg/ml.
ARL antibodies were used at a concentration of 5 to 7 μg/mL. Values represent the mean of assays done in duplicate.