Summary of the results of TGF-β1 and TGF-β2 ELISAs
| Vector . | Aprotinin (50 μg/mL) . | Active TGF-β (pg/mL) . | Total TGF-β (pg/mL) . | ||
|---|---|---|---|---|---|
| TGF-β1 . | TGF-β2 . | TGF-β1 . | TGF-β2 . | ||
| pClneo | − | 20.2 ± 3.3 | 18.1 ± 3.0 | 850 ± 64 | 1035 ± 65 |
| pClneo | + | 15.2 ± 2.2 | 14.7 ± 2.0 | 963 ± 47 | 851 ± 126 |
| Zf9-pClneo | − | 65.3 ± 7.2* | 48.6 ± 6.5* | 1,029 ± 47* | 1,118 ± 51 |
| Zf9-pClneo | + | 37.4 ± 1.1† | 29.8 ± 1.0† | 1,041 ± 79 | 1,035 ± 53 |
| Vector . | Aprotinin (50 μg/mL) . | Active TGF-β (pg/mL) . | Total TGF-β (pg/mL) . | ||
|---|---|---|---|---|---|
| TGF-β1 . | TGF-β2 . | TGF-β1 . | TGF-β2 . | ||
| pClneo | − | 20.2 ± 3.3 | 18.1 ± 3.0 | 850 ± 64 | 1035 ± 65 |
| pClneo | + | 15.2 ± 2.2 | 14.7 ± 2.0 | 963 ± 47 | 851 ± 126 |
| Zf9-pClneo | − | 65.3 ± 7.2* | 48.6 ± 6.5* | 1,029 ± 47* | 1,118 ± 51 |
| Zf9-pClneo | + | 37.4 ± 1.1† | 29.8 ± 1.0† | 1,041 ± 79 | 1,035 ± 53 |
The concentration of active and total TGF-β1 as well as TGF-β2 in each conditioned medium prepared in Figure 6 was determined by subtype-specific ELISAs as described in “Materials and Methods.” Each value represents the average ± SD from triplicate determinations. Each experiment was repeated 3 times with similar results in all, and representative results are shown.
P values obtained by a comparison between pClneo andZf9-pClneo are less than 0.05 except for total TGF-β2 concentration.
P values obtained by a comparison betweenZf9-pClneo and Zf9-pClneo + aprotinin are less than 0.05 for both active TGF-β1 and TGF-β2 concentrations.