Production of RANTES, MIP-1, and MIP-1β by SK-specific and Der p 1–specific short-term T cell lines infected in vitro with the HIV-1 BaL strain
| Ag Specificity . | No. of T Cell Lines . | Chemokine Concentration (pg/mL) . | ||
|---|---|---|---|---|
| RANTES . | MIP-1α . | MIP-1β . | ||
| SK | 8 | 285 ± 84* | 414 ± 109 | 2402 ± 1043 |
| Der p 1 | 8 | 41 ± 9 | 102 ± 8 | 98 ± 30 |
| P values | <.01 | <.01 | <.03 | |
| Ag Specificity . | No. of T Cell Lines . | Chemokine Concentration (pg/mL) . | ||
|---|---|---|---|---|
| RANTES . | MIP-1α . | MIP-1β . | ||
| SK | 8 | 285 ± 84* | 414 ± 109 | 2402 ± 1043 |
| Der p 1 | 8 | 41 ± 9 | 102 ± 8 | 98 ± 30 |
| P values | <.01 | <.01 | <.03 | |
SK-specific and Der p 1–specific short-term T cell lines were generated from PBMC of 8 Der p 1–sensitive atopic donors, as described in Materials and Methods. On day 15, T cell lines were infected with the HIV-1BaL strain, and on day 6 after infection they were assessed for the production of RANTES, MIP-1α, and MIP-1β, as described in “Materials and Methods.”
Values represent the mean ± SE of chemokine concentrations in cell-free supernatants.