Effect of acidosis induced by ammonium chloride on KG-1A leukemic cells
| Time . | No NH4Cl . | Addition of NH4Cl . | ||
|---|---|---|---|---|
| pHi . | Annexin-V . | pHi . | Annexin-V . | |
| 0 | 7.24 ± 0.02 | 4.1 ± 1.6 | — | — |
| 30 min | 7.09 ± 0.02 | 4.6 ± 2.2 | 6.86 ± 0.03 | 4.4 ± 2.9 |
| 1 h | 7.14 ± 0.04 | 4.9 ± 1.9 | 6.62 ± 0.06 | 15.9 ± 5.2 |
| 3 h | 6.98 ± 0.02 | 4.0 ± 1.7 | 6.55 ± 0.04 | 26.8 ± 8.6 |
| 5 h | 7.05 ± 0.03 | 4.1 ± 2.4 | 6.75 ± 0.05 | 12.2 ± 6.3 |
| Time . | No NH4Cl . | Addition of NH4Cl . | ||
|---|---|---|---|---|
| pHi . | Annexin-V . | pHi . | Annexin-V . | |
| 0 | 7.24 ± 0.02 | 4.1 ± 1.6 | — | — |
| 30 min | 7.09 ± 0.02 | 4.6 ± 2.2 | 6.86 ± 0.03 | 4.4 ± 2.9 |
| 1 h | 7.14 ± 0.04 | 4.9 ± 1.9 | 6.62 ± 0.06 | 15.9 ± 5.2 |
| 3 h | 6.98 ± 0.02 | 4.0 ± 1.7 | 6.55 ± 0.04 | 26.8 ± 8.6 |
| 5 h | 7.05 ± 0.03 | 4.1 ± 2.4 | 6.75 ± 0.05 | 12.2 ± 6.3 |
pHi measurements were performed by fluorescence ratio imaging microscopy (FRIM), whereas annexin-V detection was performed by flow cytometry. Results represent the mean and SEM of 2 experiments.