Table 5. Sequence variations in rare ABO... | American Society of Hematology

Table 5.

Sequence variations in rare ABO alleles identified in this study (base changes shown with reference to the A¹allele)

Allele^5-151	N^5-152	SSCP Patterns	Exon 6			Exon 7
			F1^5-150			F2^5-150															F3^5-150
			nt 261	297	318	467	526	542	579	595	646	657	681	703	771	796	802	803	829	930	1059- 1061
A¹		2b/1c/3b	G	A	C	C	C	G	T	C	T	C	G	G	C	C	G	G	G	G	C
A^v1	1	2b/1c/3c																			—
O^v1	1	2e/1a/3b	—								A		A		T				A
O^v2	5	2a/1a/3b	—		T	T
O^v3	1	2g/1a/3b	—									T
O^v4	1	2j/1a/3b	—						C
O^v5	1	2i/1b/3b	—	G				A			A		A		T				A
O^v6	5	2h/1b/3b	—	G						T	A		A		T				A
O^v7	3	2f/1b/3b	—	G							A				T				A

Allele^5-151	N^5-152	SSCP Patterns	Exon 6			Exon 7
			F1^5-150			F2^5-150															F3^5-150
			nt 261	297	318	467	526	542	579	595	646	657	681	703	771	796	802	803	829	930	1059- 1061
A¹		2b/1c/3b	G	A	C	C	C	G	T	C	T	C	G	G	C	C	G	G	G	G	C
A^v1	1	2b/1c/3c																			—
O^v1	1	2e/1a/3b	—								A		A		T				A
O^v2	5	2a/1a/3b	—		T	T
O^v3	1	2g/1a/3b	—									T
O^v4	1	2j/1a/3b	—						C
O^v5	1	2i/1b/3b	—	G				A			A		A		T				A
O^v6	5	2h/1b/3b	—	G						T	A		A		T				A
O^v7	3	2f/1b/3b	—	G							A				T				A

F5-150

F1, F2, and F3 are the 3 PCR fragments used to assay the known single nucleotide polymorphisms. Nucleotide (nt) positions shown in boldface (nt 318, 542, and 595) are new mutation sites not previously reported. Nt 1096 in F3 is not shown here because it is within the region to which the sequencing primer ABOi7R5 anneals. Note that deletions are denoted by dashes.

F5-151

O^v1 and O^v4 were found in Chinese and also previously reported in Japanese,8 whereas the rest were found in white Europeans and were new alleles.

F5-152

N refers to the number of chromosomes found for each rare allele.

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