Analyses of cell surface antigens in MRL/lpr mice treated with (5.5 Gy × 2 + PV + LV) or nontreated MRL/lpr mice
| Mice (age) . | Organs . | Cell Surface Antigens (%) . | H-2 Typing (%) . | |||
|---|---|---|---|---|---|---|
| Thy 1.2+/ B220− . | Thy 1.2−/ B220+ . | Thy 1.2+/ B220+ . | H-2k . | H-2b . | ||
| Treated MRL/lpr (64 wk) | Spleen | 13.2 ± 8.8 | 60.0 ± 14.9 | 0.3 ± 0.2 | 0.0 ± 0.0 | 97.9 ± 2.3 |
| Lymph nodes | 26.6 ± 0.0 | 45.6 ± 5.2 | 0.8 ± 0.2 | 0.4 ± 0.5 | 93.7 ± 4.2 | |
| Nontreated MRL/lpr (20 wk) | Spleen | 30.2 ± 8.5 | 26.7 ± 11.5 | 24.7 ± 11.0 | 99.3 ± 0.6 | 0.0 ± 0.0 |
| Lymph nodes | 27.0 ± 4.6 | 7.5 ± 2.1 | 62.1 ± 23.6 | 99.9 ± 0.2 | 0.0 ± 0.0 | |
| B6 (12 wk) | Spleen | 19.2 ± 2.0 | 71.2 ± 3.6 | 0.8 ± 0.18 | 0.0 ± 0.0 | 99.7 ± 0.1 |
| Lymph nodes | 66.5 ± 9.7 | 29.2 ± 7.0 | 1.3 ± 0.4 | 0.0 ± 0.0 | 99.8 ± 0.1 | |
| Mice (age) . | Organs . | Cell Surface Antigens (%) . | H-2 Typing (%) . | |||
|---|---|---|---|---|---|---|
| Thy 1.2+/ B220− . | Thy 1.2−/ B220+ . | Thy 1.2+/ B220+ . | H-2k . | H-2b . | ||
| Treated MRL/lpr (64 wk) | Spleen | 13.2 ± 8.8 | 60.0 ± 14.9 | 0.3 ± 0.2 | 0.0 ± 0.0 | 97.9 ± 2.3 |
| Lymph nodes | 26.6 ± 0.0 | 45.6 ± 5.2 | 0.8 ± 0.2 | 0.4 ± 0.5 | 93.7 ± 4.2 | |
| Nontreated MRL/lpr (20 wk) | Spleen | 30.2 ± 8.5 | 26.7 ± 11.5 | 24.7 ± 11.0 | 99.3 ± 0.6 | 0.0 ± 0.0 |
| Lymph nodes | 27.0 ± 4.6 | 7.5 ± 2.1 | 62.1 ± 23.6 | 99.9 ± 0.2 | 0.0 ± 0.0 | |
| B6 (12 wk) | Spleen | 19.2 ± 2.0 | 71.2 ± 3.6 | 0.8 ± 0.18 | 0.0 ± 0.0 | 99.7 ± 0.1 |
| Lymph nodes | 66.5 ± 9.7 | 29.2 ± 7.0 | 1.3 ± 0.4 | 0.0 ± 0.0 | 99.8 ± 0.1 | |
The results are expressed as the mean ± SD of 6 mice. Cells from the spleen and lymph nodes (inguinal and axillary regions) were stained with FITC- or PE-conjugated mAbs, and analyzed using a FACScan.