Table 1. Oligonucleotide primers used in... | American Society of Hematology

Table 1.

Oligonucleotide primers used in ASO hybridizations and in site-directed mutagenesis

Primer	Sequence	Position^*	Location (FGB exon)
FGB-L353^†	5′ TAATGCCCTCATGGATG 3′	7148-7164	7
FGB-R353^†	5′ TAATGCCCGCATGGATG 3′	7148-7164	7
FGB-G400^†	5′ AGACGGTGGTGGATGGT 3′	7907-7923	8
FGB-D400^†	5′ AGACGGTGATGGATGGT 3′	7907-7923	8
L353R-F^‡	5′ CCGGTAATGCCCGCATGGATGGAGC 3′	7144-7168	7
L353R-R^‡	5′ GCTCCATCCATGCGGGCATTACCGG 3′	7168-7144	7
G400D-F^‡	5′ AAGAAGACGGTGATGGATGGTGGTA 3′	7903-7927	8
G400D-R^‡	5′ TACCACCATCCATCACCGTCTTCTT 3′	7927-7903	8

Primer	Sequence	Position^*	Location (FGB exon)
FGB-L353^†	5′ TAATGCCCTCATGGATG 3′	7148-7164	7
FGB-R353^†	5′ TAATGCCCGCATGGATG 3′	7148-7164	7
FGB-G400^†	5′ AGACGGTGGTGGATGGT 3′	7907-7923	8
FGB-D400^†	5′ AGACGGTGATGGATGGT 3′	7907-7923	8
L353R-F^‡	5′ CCGGTAATGCCCGCATGGATGGAGC 3′	7144-7168	7
L353R-R^‡	5′ GCTCCATCCATGCGGGCATTACCGG 3′	7168-7144	7
G400D-F^‡	5′ AAGAAGACGGTGATGGATGGTGGTA 3′	7903-7927	8
G400D-R^‡	5′ TACCACCATCCATCACCGTCTTCTT 3′	7927-7903	8

Numbering according to Genbank accession number M64983.

Probes used for ASO hybridization.

Oligonucleotides used for site-directed mutagenesis.

FGB, fibrinogen Bβ-chain gene.