Clonal analysis of LacZ-positive cells after differentiation induction from c-ets-1+/+ and c-ets-1−/− ES cells
| Genotype of ES Cells . | Percentages of LacZ+ Cells . | ||||
|---|---|---|---|---|---|
| Megakaryocytes . | Erythrocytes . | Macrophages . | |||
| Day 8 . | Day 12 . | Day 8 . | Day 12 . | Day 10 . | |
| +/+ | 84.8 | 93.1 | <0.1 | <0.1 | <0.1 |
| +/+ | 5.0 | 2.9 | <0.1 | <0.1 | <0.1 |
| +/+ | 89.8 | 45.2 | <0.1 | <0.1 | <0.1 |
| +/+ | 66.5 | 8.3 | <0.1 | <0.1 | <0.1 |
| +/+ | 65.1 | 32.8 | <0.1 | <0.1 | <0.1 |
| +/+ | 91.0 | 96.7 | <0.1 | <0.1 | <0.1 |
| +/+ | 65.2 | 50.2 | <0.1 | <0.1 | <0.1 |
| +/+ | 67.0 | 86.3 | <0.1 | <0.1 | <0.1 |
| −/− | 85.3 | 91.0 | <0.1 | <0.1 | <0.1 |
| −/− | 89.0 | 91.0 | <0.1 | <0.1 | <0.1 |
| −/− | 95.9 | 77.1 | <0.1 | <0.1 | <0.1 |
| −/− | 51.6 | 27.6 | <0.1 | <0.1 | <0.1 |
| −/− | 80.5 | 85.5 | <0.1 | <0.1 | <0.1 |
| −/− | 80.9 | 22.5 | <0.1 | <0.1 | <0.1 |
| −/− | 67.8 | 70.7 | <0.1 | <0.1 | <0.1 |
| Genotype of ES Cells . | Percentages of LacZ+ Cells . | ||||
|---|---|---|---|---|---|
| Megakaryocytes . | Erythrocytes . | Macrophages . | |||
| Day 8 . | Day 12 . | Day 8 . | Day 12 . | Day 10 . | |
| +/+ | 84.8 | 93.1 | <0.1 | <0.1 | <0.1 |
| +/+ | 5.0 | 2.9 | <0.1 | <0.1 | <0.1 |
| +/+ | 89.8 | 45.2 | <0.1 | <0.1 | <0.1 |
| +/+ | 66.5 | 8.3 | <0.1 | <0.1 | <0.1 |
| +/+ | 65.1 | 32.8 | <0.1 | <0.1 | <0.1 |
| +/+ | 91.0 | 96.7 | <0.1 | <0.1 | <0.1 |
| +/+ | 65.2 | 50.2 | <0.1 | <0.1 | <0.1 |
| +/+ | 67.0 | 86.3 | <0.1 | <0.1 | <0.1 |
| −/− | 85.3 | 91.0 | <0.1 | <0.1 | <0.1 |
| −/− | 89.0 | 91.0 | <0.1 | <0.1 | <0.1 |
| −/− | 95.9 | 77.1 | <0.1 | <0.1 | <0.1 |
| −/− | 51.6 | 27.6 | <0.1 | <0.1 | <0.1 |
| −/− | 80.5 | 85.5 | <0.1 | <0.1 | <0.1 |
| −/− | 80.9 | 22.5 | <0.1 | <0.1 | <0.1 |
| −/− | 67.8 | 70.7 | <0.1 | <0.1 | <0.1 |
Hematopoietic cells were generated from c-ets-1+/+ and c-ets-1−/− ES cells after transfection of the GPIIb wild-type promoter-LacZ construct. The format of the table is the same as in Table 1.