Lineage-specific expression of LacZ driven by various promoters
| Promoters3-150 . | Megakaryocytes . | Erythrocytes . | Macrophages . | |
|---|---|---|---|---|
| Primitive . | Definitive . | |||
| GPIIb (−598/+33) | ++ | − | − | − |
| GPIIb deletion mutants | + ∼ +++ | − | − | − |
| GATAm | + | − | − | − |
| ETSm | + | − | − | − |
| GPIIb −598/−400:TK | ++ | − | − | − |
| GPIIb −539/−420:TK | + | − | − | − |
| GPIIb −529/−450:TK | + | − | − | − |
| GPIIb −400/−598:TK | + | − | − | − |
| GPIIb TK:−598/−400 | + | − | − | − |
| 4 × GATA:TK | + | + | + | − |
| 4 × ETS:TK | ± | − | − | − |
| EF-1α | + ∼ ++ | + ∼ ++ | + ∼ ++ | + ∼ ++ |
| Promoters3-150 . | Megakaryocytes . | Erythrocytes . | Macrophages . | |
|---|---|---|---|---|
| Primitive . | Definitive . | |||
| GPIIb (−598/+33) | ++ | − | − | − |
| GPIIb deletion mutants | + ∼ +++ | − | − | − |
| GATAm | + | − | − | − |
| ETSm | + | − | − | − |
| GPIIb −598/−400:TK | ++ | − | − | − |
| GPIIb −539/−420:TK | + | − | − | − |
| GPIIb −529/−450:TK | + | − | − | − |
| GPIIb −400/−598:TK | + | − | − | − |
| GPIIb TK:−598/−400 | + | − | − | − |
| 4 × GATA:TK | + | + | + | − |
| 4 × ETS:TK | ± | − | − | − |
| EF-1α | + ∼ ++ | + ∼ ++ | + ∼ ++ | + ∼ ++ |
The absolute percentages of LacZ-positive cells produced with different promoters in megakaryocytes, erythroid cells, and macrophages are shown. − indicates negative; ± indicates very low (<0.5%); + indicates low (0.5% ∼ 15%); ++ indicates medium (15% ∼ 25%); +++ indicates high (>25%).
The names of the promoters are explained in the text.