Analysis of LacZ-positive cells as pools after differentiation induction
| Promoter* . | Percentages of LacZ+ Cells . | ||||
|---|---|---|---|---|---|
| Megakaryocytes . | Erythrocytes . | Macrophages . | |||
| Day 8 . | Day 12 . | Day 8 . | Day 12 . | Day 10 . | |
| GPIIb (−598/+33) | 32.3 33.2 | 15.4 21.4 | <0.1 <0.1 | <0.1 <0.1 | <0.1 <0.1 |
| 32.9 | 16.8 | <0.1 | <0.1 | <0.1 | |
| 27.7 | 12.9 | <0.1 | <0.1 | <0.1 | |
| 35.4 | 18.8 | <0.1 | <0.1 | <0.1 | |
| 28.8 | 16.3 | <0.1 | <0.1 | <0.1 | |
| GPIIb (−100/+33) | <0.1 0.8 | <0.1 0.5 | <0.1 <0.1 | <0.1 <0.1 | <0.1 <0.1 |
| 3.9 | 1.0 | <0.1 | <0.1 | <0.1 | |
| <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | |
| <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | |
| <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | |
| GPIIb (−598/−400: −100/+33) | 41.2 21.0 25.0 | 23.1 12.8 16.2 | <0.1 <0.1 <0.1 | <0.1 <0.1 <0.1 | <0.1 <0.1 <0.1 |
| 25.0 | 10.3 | <0.1 | <0.1 | <0.1 | |
| 19.1 | 12.3 | <0.1 | <0.1 | <0.1 | |
| 33.3 | 25.0 | <0.1 | <0.1 | <0.1 | |
| Promoter* . | Percentages of LacZ+ Cells . | ||||
|---|---|---|---|---|---|
| Megakaryocytes . | Erythrocytes . | Macrophages . | |||
| Day 8 . | Day 12 . | Day 8 . | Day 12 . | Day 10 . | |
| GPIIb (−598/+33) | 32.3 33.2 | 15.4 21.4 | <0.1 <0.1 | <0.1 <0.1 | <0.1 <0.1 |
| 32.9 | 16.8 | <0.1 | <0.1 | <0.1 | |
| 27.7 | 12.9 | <0.1 | <0.1 | <0.1 | |
| 35.4 | 18.8 | <0.1 | <0.1 | <0.1 | |
| 28.8 | 16.3 | <0.1 | <0.1 | <0.1 | |
| GPIIb (−100/+33) | <0.1 0.8 | <0.1 0.5 | <0.1 <0.1 | <0.1 <0.1 | <0.1 <0.1 |
| 3.9 | 1.0 | <0.1 | <0.1 | <0.1 | |
| <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | |
| <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | |
| <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | |
| GPIIb (−598/−400: −100/+33) | 41.2 21.0 25.0 | 23.1 12.8 16.2 | <0.1 <0.1 <0.1 | <0.1 <0.1 <0.1 | <0.1 <0.1 <0.1 |
| 25.0 | 10.3 | <0.1 | <0.1 | <0.1 | |
| 19.1 | 12.3 | <0.1 | <0.1 | <0.1 | |
| 33.3 | 25.0 | <0.1 | <0.1 | <0.1 | |
LacZ-positive cells from individual wells as pools were analyzed after differentiation induction. The experiment was similar to that in Table 1.