Table 5. Multilineage NGFR expression on... | American Society of Hematology

Table 5.

Multilineage NGFR expression on peripheral blood cells after secondary BMT with sorted NGFR⁺c-kit⁺ Sca-1⁺ cells

	Estimated Number of NGFR⁺ c-kit⁺ Sca-1⁺Cells Injected	Mouse #	% Ly5.2⁺	% NGFR⁺ On
	Estimated Number of NGFR⁺ c-kit⁺ Sca-1⁺Cells Injected	Mouse #	% Ly5.2⁺	Ly5.2⁺ WBCs	RBCs	Platelets
Experiment 1 (5/5 engrafted)	1900	1	52.4	52.6	33.9	23.1
	1900	2	27.1	30.1	15.4	22.7
	1900	3	35.4	51.8	40.9	35.7
	1900	4	22.1	59	31.4	9.5
	1900	5	12.4	16.7	17.2	4.4
	2 × 10⁵ BM injected	6	11.2	2.2	24.2	5.8
	2 × 10⁵BM injected	7	73.9	0.5	1	1.1
	Control	BA.1	99.4	0.4	0.2	0.4
	Control	B6SJL	0	0	0	0.5
Experiment 2 (3/5 engrafted)	100	1	12.4	47.6	4.9	6
	100	2	51.8	13.4	21.8	12.7
	100	3	25.1	60.7	18	31
	Control	BA.1	94.4	0.2	0	0.7
	Control	B6SJL	0.3	0	0	0.5

	Estimated Number of NGFR⁺ c-kit⁺ Sca-1⁺Cells Injected	Mouse #	% Ly5.2⁺	% NGFR⁺ On
	Estimated Number of NGFR⁺ c-kit⁺ Sca-1⁺Cells Injected	Mouse #	% Ly5.2⁺	Ly5.2⁺ WBCs	RBCs	Platelets
Experiment 1 (5/5 engrafted)	1900	1	52.4	52.6	33.9	23.1
	1900	2	27.1	30.1	15.4	22.7
	1900	3	35.4	51.8	40.9	35.7
	1900	4	22.1	59	31.4	9.5
	1900	5	12.4	16.7	17.2	4.4
	2 × 10⁵ BM injected	6	11.2	2.2	24.2	5.8
	2 × 10⁵BM injected	7	73.9	0.5	1	1.1
	Control	BA.1	99.4	0.4	0.2	0.4
	Control	B6SJL	0	0	0	0.5
Experiment 2 (3/5 engrafted)	100	1	12.4	47.6	4.9	6
	100	2	51.8	13.4	21.8	12.7
	100	3	25.1	60.7	18	31
	Control	BA.1	94.4	0.2	0	0.7
	Control	B6SJL	0.3	0	0	0.5

Each mouse was lethally irradiated and transplanted with sorted NGFR⁺ c-kit⁺ Sca-1⁺ cells pooled from donors shown in Table 4, as described in “Materials and Methods.” Peripheral blood analysis was performed as in Figure 1. Data shown are the percentage of Ly-5.2⁺ or NGFR⁺ cells in peripheral blood of individual mice 2 months after BMT.

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