Transgene expression on bone marrow stem/progenitor cells
| Mouse # . | % NGFR+ On . | Estimated Number of NGFR+c-kit+ Sca-1+ Cells . | |||
|---|---|---|---|---|---|
| WBCs . | BM Lin−/lo Sca-1+ Cells . | BM c-kit+ Sca-1+ Cells . | In BM of Long Bones . | Injected/ms After Sorting . | |
| 017 | 7.6 | 0.6 | N/D | ||
| 023 | 11.2 | 1.3 | N/D | ||
| 014 | 12.2 | 3.7 | N/D | ||
| 011 | 33.6 | 22.2 | N/D | ||
| 211 | 24.7 | N/D | 6.7 | 3425 | N/D |
| 213 | 44.3 | N/D | 22.7 | 5461 | N/D |
| 217 | 26.6 | N/D | 7.2 | 18538 | N/D |
| 221 | 6.3 | N/D | (pooled with 222) | ||
| 222 | 29.0 | N/D | 10.2 | 9644 | 1900 |
| 015 | 19.0 | N/D | (pooled with 227) | ||
| 227 | 21.4 | N/D | 13.2 | 6300 | 100 |
| Mouse # . | % NGFR+ On . | Estimated Number of NGFR+c-kit+ Sca-1+ Cells . | |||
|---|---|---|---|---|---|
| WBCs . | BM Lin−/lo Sca-1+ Cells . | BM c-kit+ Sca-1+ Cells . | In BM of Long Bones . | Injected/ms After Sorting . | |
| 017 | 7.6 | 0.6 | N/D | ||
| 023 | 11.2 | 1.3 | N/D | ||
| 014 | 12.2 | 3.7 | N/D | ||
| 011 | 33.6 | 22.2 | N/D | ||
| 211 | 24.7 | N/D | 6.7 | 3425 | N/D |
| 213 | 44.3 | N/D | 22.7 | 5461 | N/D |
| 217 | 26.6 | N/D | 7.2 | 18538 | N/D |
| 221 | 6.3 | N/D | (pooled with 222) | ||
| 222 | 29.0 | N/D | 10.2 | 9644 | 1900 |
| 015 | 19.0 | N/D | (pooled with 227) | ||
| 227 | 21.4 | N/D | 13.2 | 6300 | 100 |
Bone marrow (BM) was recovered from 4 long bones of each mouse and analyzed by flow cytometry or sorted as described in “Materials and Methods.” Data represent the percentage of NGFR+live-gated peripheral blood WBCs or BM cells analyzed as in Figures 1and 5. The number of NGFR+ c-kit+Sca-1+ cells from bone marrow of individual mice or injected per mouse (ms) after sorting was calculated using the gating criteria shown in Figure 5.