Transgene expression on myeloid and lymphoid phenotypic subsets of splenocytes
Mouse# . | % Ly5.2+ . | % NGFR+ on Ly5.2+Splenocytes . | |||||
---|---|---|---|---|---|---|---|
Total . | Gr-1+ . | Mac-1+ . | B220+ . | CD4+ . | CD8+ . | ||
BA.1 control | 97.8 | 0.2 | 0.2 | 0 | 0.2 | 0 | 0 |
B6SJL | 0.03 | 0 | 0 | 0 | 0 | 0 | 0 |
Mock | 96.3 | 0.4 | 0.2 | 0.1 | 0.1 | 0.1 | 1.5 |
#211 | 90.8 | 7.1 | 17.2 | 14.3 | 4.4 | 10.2 | 13.9 |
#213 | 92.5 | 47.4 | 60.4 | 47.3 | 48 | 53.7 | 36.8 |
#217 | 91.3 | 16.9 | 29.6 | 22.2 | 13.8 | 10.3 | 15 |
Mouse# . | % Ly5.2+ . | % NGFR+ on Ly5.2+Splenocytes . | |||||
---|---|---|---|---|---|---|---|
Total . | Gr-1+ . | Mac-1+ . | B220+ . | CD4+ . | CD8+ . | ||
BA.1 control | 97.8 | 0.2 | 0.2 | 0 | 0.2 | 0 | 0 |
B6SJL | 0.03 | 0 | 0 | 0 | 0 | 0 | 0 |
Mock | 96.3 | 0.4 | 0.2 | 0.1 | 0.1 | 0.1 | 1.5 |
#211 | 90.8 | 7.1 | 17.2 | 14.3 | 4.4 | 10.2 | 13.9 |
#213 | 92.5 | 47.4 | 60.4 | 47.3 | 48 | 53.7 | 36.8 |
#217 | 91.3 | 16.9 | 29.6 | 22.2 | 13.8 | 10.3 | 15 |
Spleens from individual mice were recovered 8 months after transplantation, and splenocytes were analyzed by flow cytometry as described in “Materials and Methods.” Data represent the percentage of Ly-5.2+ or Ly-5.2+NGFR+ splenocytes. All other abbreviations are as in Figure1.