Transgene expression on thymocytes at various stages of maturation
| Mouse# . | % Ly5.2+ . | % NGFR+ on Ly5.2+ Thymocytes . | |||
|---|---|---|---|---|---|
| Total . | CD4 + CD8 + DP . | CD4 + SP . | CD8 + SP . | ||
| BA.1 control | 99.7 | 0.8 | 0.1 | 0 | 0.1 |
| B6SJL | 0.2 | 0 | 0.1 | 0 | 0.3 |
| Mock | 98.3 | 0.3 | 0 | 0 | 0.3 |
| #211 | 80.8 | 13.8 | 8.5 | 7.2 | 59.9 |
| #213 | 91.2 | 37.1 | 0 | 52.6 | 15 |
| #217 | 88.8 | 33.4 | 63.1 | 35.6 | 33.7 |
| Mouse# . | % Ly5.2+ . | % NGFR+ on Ly5.2+ Thymocytes . | |||
|---|---|---|---|---|---|
| Total . | CD4 + CD8 + DP . | CD4 + SP . | CD8 + SP . | ||
| BA.1 control | 99.7 | 0.8 | 0.1 | 0 | 0.1 |
| B6SJL | 0.2 | 0 | 0.1 | 0 | 0.3 |
| Mock | 98.3 | 0.3 | 0 | 0 | 0.3 |
| #211 | 80.8 | 13.8 | 8.5 | 7.2 | 59.9 |
| #213 | 91.2 | 37.1 | 0 | 52.6 | 15 |
| #217 | 88.8 | 33.4 | 63.1 | 35.6 | 33.7 |
Thymocytes from individual mice were recovered 8 months after transplantation and analyzed by flow cytometry as described in “Materials and Methods.” Data represent the percentage of Ly-5.2+ or Ly-5.2+ NGFR+thymocytes. DP, double positives; SP, single positives. In mouse #213, the percentage Ly-5.2+ NGFR+ in DP is below detectable levels because DP were less than 0.1% of total thymocytes. All other abbreviations are as in Figure 1.