Table 1. Enduring multilineage... | American Society of Hematology

Table 1.

Enduring multilineage hematopoiesis reconstituted from transgene-expressing cells after transplantation with transduced bone marrow

Days After Transplant	Mouse#	% Ly5.2⁺	% NGFR⁺ on Ly-5.2⁺ WBCs					% NGFR⁺ On
Days After Transplant	Mouse#	% Ly5.2⁺	Total	Gr-1⁺	Mac-1⁺	B220⁺	CD3e⁺	RBCs	Platelets
Experiment 1, day 174	BA.1 control	98.2 ± 1.3	0.3 ± 0.0	1.1 ± 0.3	1.1 ± 0.2	0.8 ± 0.2	1.7 ± 0.6	0	0
	Mock	94.8 ± 1.1	0.3 ± 0.1	1.0 ± 0.4	0.9 ± 0.2	1.2 ± 0.3	1.0 ± 0.9	0	0
	1171 (n = 8)	93.8 ± 1.0	12.6 ± 9.7	36.1 ± 24.9	28.2 ± 19.5	13.2 ± 6.6	11.6 ± 7.1	10.6 ± 21.0	8.7 ± 14.8
	1171 Range	92.7-95.5	3.7-33.6	5.1-72.3	6.3-61.5	2.9-24.3	3.5-24.3	0.9-62.3	0.8-44.7
Experiment 2, day 161	BA.1 control	94.2 ± 8.6	0.3 ± 0.0	0.8 ± 0.3	0.8 ± 0.2	0.5 ± 0.0	0.6 ± 0.2	0	0
	Mock	95.6 ± 0.9	0.4 ± 0.1	1.0 ± 0.4	0.9 ± 0.3	0.6 ± 0.2	0.5 ± 0.2	0	0
1171 (n = 7)	90.0 ± 5.7	20.2 ± 9.2	51.6 ± 12.5	44.0 ± 12.0	13.2 ± 8.5	14.5 ± 7.3	14.1 ± 14.0	15.7 ± 11.7
	1171 Range	79.2-94.8	11.9-38.9	35.4-73.1	30.2-67.1	7.2-30.4	7.4-28.1	4.1-39.9	6.6-35.6
Experiment 2, day 249	BA.1 control	98.5	0.4	0.2	0.6	0.2	0.5	0	0
	B6SJL	0	0	0	0	0	0	0.2	0.1
	Mock	96.1	0.3	0.3	1.2	0.5	0.2	0	0.1
	#211	90.2	24.7	71.9	57.8	9.5	16.8	8.6	10.3
	#213	86.4	44.3	65.2	47.4	37.4	20.3	17.9	19.8
	#217	92.4	26.6	53.7	47.3	13.1	17.4	17.1	16.9

Days After Transplant	Mouse#	% Ly5.2⁺	% NGFR⁺ on Ly-5.2⁺ WBCs					% NGFR⁺ On
Days After Transplant	Mouse#	% Ly5.2⁺	Total	Gr-1⁺	Mac-1⁺	B220⁺	CD3e⁺	RBCs	Platelets
Experiment 1, day 174	BA.1 control	98.2 ± 1.3	0.3 ± 0.0	1.1 ± 0.3	1.1 ± 0.2	0.8 ± 0.2	1.7 ± 0.6	0	0
	Mock	94.8 ± 1.1	0.3 ± 0.1	1.0 ± 0.4	0.9 ± 0.2	1.2 ± 0.3	1.0 ± 0.9	0	0
	1171 (n = 8)	93.8 ± 1.0	12.6 ± 9.7	36.1 ± 24.9	28.2 ± 19.5	13.2 ± 6.6	11.6 ± 7.1	10.6 ± 21.0	8.7 ± 14.8
	1171 Range	92.7-95.5	3.7-33.6	5.1-72.3	6.3-61.5	2.9-24.3	3.5-24.3	0.9-62.3	0.8-44.7
Experiment 2, day 161	BA.1 control	94.2 ± 8.6	0.3 ± 0.0	0.8 ± 0.3	0.8 ± 0.2	0.5 ± 0.0	0.6 ± 0.2	0	0
	Mock	95.6 ± 0.9	0.4 ± 0.1	1.0 ± 0.4	0.9 ± 0.3	0.6 ± 0.2	0.5 ± 0.2	0	0
1171 (n = 7)	90.0 ± 5.7	20.2 ± 9.2	51.6 ± 12.5	44.0 ± 12.0	13.2 ± 8.5	14.5 ± 7.3	14.1 ± 14.0	15.7 ± 11.7
	1171 Range	79.2-94.8	11.9-38.9	35.4-73.1	30.2-67.1	7.2-30.4	7.4-28.1	4.1-39.9	6.6-35.6
Experiment 2, day 249	BA.1 control	98.5	0.4	0.2	0.6	0.2	0.5	0	0
	B6SJL	0	0	0	0	0	0	0.2	0.1
	Mock	96.1	0.3	0.3	1.2	0.5	0.2	0	0.1
	#211	90.2	24.7	71.9	57.8	9.5	16.8	8.6	10.3
	#213	86.4	44.3	65.2	47.4	37.4	20.3	17.9	19.8
	#217	92.4	26.6	53.7	47.3	13.1	17.4	17.1	16.9

Data for day 174 or 161 represent the mean percentage ± SD of Ly-5.2⁺ or NGFR⁺ cells in peripheral blood of mice after transplantation of bone marrow cells transduced with the 1171 vector. Data for day 249 show the percentage of Ly-5.2⁺ or NGFR⁺ cells in various peripheral blood cell lineages of 3 mice. All abbreviations are as in Figure 1.

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