Table 2.

CFU-C generated from TER119(−) bone marrow cells separated according to FDG positivity

CFU ± SEM/105Cells
BFUE CFU-Mk CFU-GM Total CFC Total CFC “blue”
Bone marrow #1* 
 Pre-sort  10 ± 1.8 16 ± 2.3  306 ± 7.6  332 ± 8.7  8 ± 0.7 
 FDG ++ (9.0)  15 ± 2.1  3 ± 0.6  TC†  7 ± 0.5  
 FDG +/− (35.0)  64 ± 4.7 31 ± 3.4  411 ± 29.3  506 ± 9.2  61 ± 4.8 
 FDG neg (32.0)  17 ± 2.1  3 ± 0.2  56 ± 1.8 76 ± 3.8  13 ± 1.1  
Bone marrow #2* 
 Pre-sort 28 ± 2.1  24 ± 1.7  258 ± 3.8  320 ± 5.1 32 ± 1.8  
 FDG ++ (10.5)  31 ± 1.8  29 ± 2.3 733 ± 35.3  793 ± 40.2  33 ± 3.2  
 FDG + (37.0)  210 ± 5.9  64 ± 4.6  1270 ± 40.8 1543 ± 50.2  202 ± 7.5  
 FDG neg (27.0) 35.5  17.5 ± 2.8  587 ± 39.7 640 ± 35.7  36 ± 4.6  
Bone marrow #3* 
 Pre-sort    (No Epo used in  these cultures.)  350 ± 19    
 FDG ++ (2.0)   380 ± 39  
 FDG + (39.0)    248 ± 21 
 FDG neg (55.0)    114 ± 17  
Bone marrow #4* 
 Pre-sort     179 ± 3.0  45 ± 3.0  
 FDG ++ (13.3)     603 ± 7.5  165 ± 5.4  
 FDG +/− (46.0)     112 ± 5.0  0 
CFU ± SEM/105Cells
BFUE CFU-Mk CFU-GM Total CFC Total CFC “blue”
Bone marrow #1* 
 Pre-sort  10 ± 1.8 16 ± 2.3  306 ± 7.6  332 ± 8.7  8 ± 0.7 
 FDG ++ (9.0)  15 ± 2.1  3 ± 0.6  TC†  7 ± 0.5  
 FDG +/− (35.0)  64 ± 4.7 31 ± 3.4  411 ± 29.3  506 ± 9.2  61 ± 4.8 
 FDG neg (32.0)  17 ± 2.1  3 ± 0.2  56 ± 1.8 76 ± 3.8  13 ± 1.1  
Bone marrow #2* 
 Pre-sort 28 ± 2.1  24 ± 1.7  258 ± 3.8  320 ± 5.1 32 ± 1.8  
 FDG ++ (10.5)  31 ± 1.8  29 ± 2.3 733 ± 35.3  793 ± 40.2  33 ± 3.2  
 FDG + (37.0)  210 ± 5.9  64 ± 4.6  1270 ± 40.8 1543 ± 50.2  202 ± 7.5  
 FDG neg (27.0) 35.5  17.5 ± 2.8  587 ± 39.7 640 ± 35.7  36 ± 4.6  
Bone marrow #3* 
 Pre-sort    (No Epo used in  these cultures.)  350 ± 19    
 FDG ++ (2.0)   380 ± 39  
 FDG + (39.0)    248 ± 21 
 FDG neg (55.0)    114 ± 17  
Bone marrow #4* 
 Pre-sort     179 ± 3.0  45 ± 3.0  
 FDG ++ (13.3)     603 ± 7.5  165 ± 5.4  
 FDG +/− (46.0)     112 ± 5.0  0 
*

Numbers in parentheses represent the gated portion sorted expressed as percentage of total cells (see example in Figure 4, #2). Note that the totals are less than 100.

†

Too crowded to count.

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