Table 1. Effect of staurosporine and... | American Society of Hematology

Table 1.

Effect of staurosporine and calphostin C on cell number in liquid cultures

Treatment			EPO					EPO + SCF
			+Staurosporine		+Calphostin C			+Staurosporine		+Calphostin C
Medium			50 nmol/L	20 nmol/L	50 nmol/L	20 nmol/L		50 nM	20 nM	50 nM	20 nM
Total number cells × 10⁶	0.34 ± 0.05	0.71 ± 0.05	0.57 ± 0.03	0.73 ± 0.07	0.59 ± 0.05	0.70 ± 0.08	1.37 ± 0.05	1.42 ± 0.11	1.53 ± 0.15	1.34 ± 0.07	1.38 ± 0.02

Treatment			EPO					EPO + SCF
			+Staurosporine		+Calphostin C			+Staurosporine		+Calphostin C
Medium			50 nmol/L	20 nmol/L	50 nmol/L	20 nmol/L		50 nM	20 nM	50 nM	20 nM
Total number cells × 10⁶	0.34 ± 0.05	0.71 ± 0.05	0.57 ± 0.03	0.73 ± 0.07	0.59 ± 0.05	0.70 ± 0.08	1.37 ± 0.05	1.42 ± 0.11	1.53 ± 0.15	1.34 ± 0.07	1.38 ± 0.02

CD34+ cells (200 000/well, 1 well/group) were cultured in medium alone or in the presence of EPO or EPO and SCF in the presence or absence of staurosporine or calphostin C. The total cell number was counted after 4 days of culture. Mean ± SEM of three separate experiments.

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