Oligonucleotides used for PCR amplification
| Name . | Sequence 5′-3′ . | Position . |
|---|---|---|
| 5MFA | GGC TCC TAA ATT CTT GGT CTC A | 92291-92312 HSIMMDL |
| 5MRA | TCC CTC TAG ATA ACA GTC ATC A | 92140-92118 HSIMMDL |
| 5MFB | GGC AAT GAG ATG GCT TTA GCT GA | 92498-92520 HSIMMDL |
| 5MRB | CTC GTG CGA CCT CTC CTT CAA A | 91910-91888 HSIMMDL |
| 3MFA | CAG TCA GGC CTC AGA GTG CA | 4095-4115 HSJHCMU |
| 3MRA | CCG CAG GCA GCC AAT AGA GT | 3931-3911 HSJHCMU |
| 3MFB | CAA GTA GTG AGG GCA CTC AGA ACG | 4353-4377 HSJHCMU |
| 3MRB | GTG ATG GGG AAC GCA GTG TAG A | 3803-3781 HSJHCMU |
| JHF | TCT CCT GCC AAG AGA GCC CC | 88884-88903 HSIMMDL |
| Name . | Sequence 5′-3′ . | Position . |
|---|---|---|
| 5MFA | GGC TCC TAA ATT CTT GGT CTC A | 92291-92312 HSIMMDL |
| 5MRA | TCC CTC TAG ATA ACA GTC ATC A | 92140-92118 HSIMMDL |
| 5MFB | GGC AAT GAG ATG GCT TTA GCT GA | 92498-92520 HSIMMDL |
| 5MRB | CTC GTG CGA CCT CTC CTT CAA A | 91910-91888 HSIMMDL |
| 3MFA | CAG TCA GGC CTC AGA GTG CA | 4095-4115 HSJHCMU |
| 3MRA | CCG CAG GCA GCC AAT AGA GT | 3931-3911 HSJHCMU |
| 3MFB | CAA GTA GTG AGG GCA CTC AGA ACG | 4353-4377 HSJHCMU |
| 3MRB | GTG ATG GGG AAC GCA GTG TAG A | 3803-3781 HSJHCMU |
| JHF | TCT CCT GCC AAG AGA GCC CC | 88884-88903 HSIMMDL |
HSIMMDL indicates GenBank sequence of the human immunoglobulin D segment locus; HSJHCMU, GenBank sequence of the human IgM heavy chain switch region.