Hematological protection against MMC exposure in FANCC-transduced (−/−) bone marrow
| Parameter . | Untreated (−/−) Mice* . | Recipients of FANCC-Transduced (−/−) Cells† . | Recipients of +/+ Cells‡ . | Secondary Recipients1-153 . | |||
|---|---|---|---|---|---|---|---|
| Before MMC . | After MMC . | Before MMC . | After MMC . | Before MMC . | After MMC . | After MMC . | |
| White cells (103/mm3) | 8.10 ± 0.10 | 4.05 ± 0.45 | 11.98 ± 1.81 | 8.42 ± 1.60 | 11.05 ± 1.05 | 7.97 ± 1.31 | 7.12 ± 0.98 |
| Red cells (106/mm3) | 9.08 ± 0.23 | 4.675 ± 1.18 | 6.64 ± 1.69 | 6.99 ± 1.77 | 6.94 ± 0.18 | 8.17 ± 0.25 | 5.96 ± 0.47 |
| Hemoglobin (g/dL) | 14.80 ± 0.00 | 7.95 ± 1.95 | 13.34 ± 1.00 | 13.72 ± 0.30 | 11.30 ± 0.29 | 13.03 ± 0.09 | 10.42 ± 0.85 |
| Hematocrit (%) | 44.40 ± 0.70 | 22.5 ± 5.70 | 38.34 ± 3.34 | 42.15 ± 0.84 | 30.93 ± 1.04 | 38.13 ± 0.47 | 30.80 ± 2.49 |
| Platelets (103/mm3) | 735.0 ± 45.0 | 289 ± 121.00 | 413.60 ± 42.59 | 661.75 ± 99.82 | 454.00 ± 41.31 | 859.33 ± 134.58 | 729.20 ± 131.65 |
| Parameter . | Untreated (−/−) Mice* . | Recipients of FANCC-Transduced (−/−) Cells† . | Recipients of +/+ Cells‡ . | Secondary Recipients1-153 . | |||
|---|---|---|---|---|---|---|---|
| Before MMC . | After MMC . | Before MMC . | After MMC . | Before MMC . | After MMC . | After MMC . | |
| White cells (103/mm3) | 8.10 ± 0.10 | 4.05 ± 0.45 | 11.98 ± 1.81 | 8.42 ± 1.60 | 11.05 ± 1.05 | 7.97 ± 1.31 | 7.12 ± 0.98 |
| Red cells (106/mm3) | 9.08 ± 0.23 | 4.675 ± 1.18 | 6.64 ± 1.69 | 6.99 ± 1.77 | 6.94 ± 0.18 | 8.17 ± 0.25 | 5.96 ± 0.47 |
| Hemoglobin (g/dL) | 14.80 ± 0.00 | 7.95 ± 1.95 | 13.34 ± 1.00 | 13.72 ± 0.30 | 11.30 ± 0.29 | 13.03 ± 0.09 | 10.42 ± 0.85 |
| Hematocrit (%) | 44.40 ± 0.70 | 22.5 ± 5.70 | 38.34 ± 3.34 | 42.15 ± 0.84 | 30.93 ± 1.04 | 38.13 ± 0.47 | 30.80 ± 2.49 |
| Platelets (103/mm3) | 735.0 ± 45.0 | 289 ± 121.00 | 413.60 ± 42.59 | 661.75 ± 99.82 | 454.00 ± 41.31 | 859.33 ± 134.58 | 729.20 ± 131.65 |
MMC indicates mitomycin C.
Negative control animals were subjected to MMC treatment only.
Transplanted animals were exposed to MMC (0.3 mg/kg for 6 weeks). Peripheral blood was obtained before and after MMC exposure. Results are expressed as the mean ± SE of all experimental animals tested (n = 5). Statistical analysis using the two-tailedt test was performed and demonstrated no significant difference between experimental animals and the wild-type controls. All −/− mice (n = 3) receiving FANCA-transduced cells and MMC died secondary to pancytopenia and marrow aplasia.
Positive control animals received +/+ cells and MMC treatment.
Secondary recipients received bone marrow cells harvested from the primary recipients of FANCC-transduced cells and MMC.