Influence of different eosinophil activators on actin response, Ca++ transients, lucigenin-dependent chemiluminescence, and CD11b expression
| Stimulus . | f-Actin . | Ca++Transients . | Chemiluminescence . | CD11b . |
|---|---|---|---|---|
| Control | 1.00 (± 0.00) | 0.92 (± 0.04) | 2.43 (± 0.78) | 2.84 (± 0.50) |
| ATP | 1.59 (± 0.07) | 2.55 (± 0.17) | 310.85 (± 65.28) | 12.15 (± 0.48) |
| Eotaxin | 1.47 (± 0.06) | 2.13 (± 0.19) | 181.20 (± 19.29) | 11.60 (± 0.73) |
| RANTES | 1.50 (± 0.04) | 2.06 (± 0.13) | 165.57 (± 24.93) | 10.61 (± 0.24) |
| C5a | 1.51 (± 0.06) | 2.39 (± 0.11) | 160.85 (± 27.28) | 11.03 (± 0.33) |
| PAF | 1.47 (± 0.04) | 2.31 (± 0.08) | 213.55 (± 23.05) | 11.97 (± 0.61) |
| Stimulus . | f-Actin . | Ca++Transients . | Chemiluminescence . | CD11b . |
|---|---|---|---|---|
| Control | 1.00 (± 0.00) | 0.92 (± 0.04) | 2.43 (± 0.78) | 2.84 (± 0.50) |
| ATP | 1.59 (± 0.07) | 2.55 (± 0.17) | 310.85 (± 65.28) | 12.15 (± 0.48) |
| Eotaxin | 1.47 (± 0.06) | 2.13 (± 0.19) | 181.20 (± 19.29) | 11.60 (± 0.73) |
| RANTES | 1.50 (± 0.04) | 2.06 (± 0.13) | 165.57 (± 24.93) | 10.61 (± 0.24) |
| C5a | 1.51 (± 0.06) | 2.39 (± 0.11) | 160.85 (± 27.28) | 11.03 (± 0.33) |
| PAF | 1.47 (± 0.04) | 2.31 (± 0.08) | 213.55 (± 23.05) | 11.97 (± 0.61) |
Eosinophils were stimulated with or without optimal concentrations of 100 μmol/mL ATP, 100 ng/mL eotaxin, 100 ng/mL RANTES, 100 nmol/L C5a, and 100 nmol/L PAF. The relative f-actin content and the ratio of the intracellular Ca++ measurements were taken after 25 seconds. Chemiluminescence response is given as integral (counts ×106) after 60 minutes. The mean channel number of CD11b expression was analyzed after 30 minutes. Data are means ± SEM (n = 5).