Effect of steel factor plus interleukin-11 on the growth of single-sorted stem cells
| . | Number of Wells (and % Positive) With at Least 2 Cells per Well at: . | |
|---|---|---|
| 5 days . | 10 days . | |
| 2-day 5FULin(−)Sca-1(+)c-kit(+) | 45/96 (46.9) | 122/288 (42.4) |
| NBMLin(−)Sca-1(+)c-kit(+)CD34(−) | 41/96 (42.7) | 40/96 (41.7) |
| Number of Wells (and % Positive) With at Least 50 Cells per Well at: | ||
| 5 days | 10 days | |
| 2-day 5FULin(−)Sca-1(+)c-kit(+) | 21/96 (21.9) | 108/288 (37.5) |
| NBMLin(−)Sca-1(+)c-kit(+)CD34(−) | 21/96 (21.9) | 34/96 (35.4) |
| . | Number of Wells (and % Positive) With at Least 2 Cells per Well at: . | |
|---|---|---|
| 5 days . | 10 days . | |
| 2-day 5FULin(−)Sca-1(+)c-kit(+) | 45/96 (46.9) | 122/288 (42.4) |
| NBMLin(−)Sca-1(+)c-kit(+)CD34(−) | 41/96 (42.7) | 40/96 (41.7) |
| Number of Wells (and % Positive) With at Least 50 Cells per Well at: | ||
| 5 days | 10 days | |
| 2-day 5FULin(−)Sca-1(+)c-kit(+) | 21/96 (21.9) | 108/288 (37.5) |
| NBMLin(−)Sca-1(+)c-kit(+)CD34(−) | 21/96 (21.9) | 34/96 (35.4) |
Single-sorted 2-day 5FU Lin(−)Sca-1(+)c-kit(+) or NBMLin(−)Sca-1(+)c-kit(+)CD34(−) cells were grown in liquid culture containing 20% FCS, 100 ng/mL rmSF, and 100 ng/mL rhIL-11 in round-bottom microtiter plates. After 5 and 10 days of incubation, cell number per well was scored using an inverted light microscope. No growth was observed in rmSF alone or rhIL-11 alone.