Characteristics of hematopoietic cell populations
| Cell Population . | HPP-CFC per 1000 . | Lymphohematopoietic Potential . |
|---|---|---|
| Normal bone marrow (NBM) | 0.50 ± 0.06 | ND |
| NBM Lin(−) | 97 ± 10 | + |
| NBM Lin(−)Sca-1(−)c-kit(+) | 96 ± 7 | − |
| NBM Lin(−)Sca-1(+)c-kit(+) | 415 ± 6 | + |
| 2-day 5 FU BM Lin(−)Sca-1(+)c-kit(+) | 194 ± 30 | + |
| NBM Lin(−)Sca-1(+)c-kit(+)CD34(+) | 15 ± 7 | ND |
| NBM Lin(−)Sca-1(+)c-kit(+)CD34(−) | 191 ± 64 | ND |
| Cell Population . | HPP-CFC per 1000 . | Lymphohematopoietic Potential . |
|---|---|---|
| Normal bone marrow (NBM) | 0.50 ± 0.06 | ND |
| NBM Lin(−) | 97 ± 10 | + |
| NBM Lin(−)Sca-1(−)c-kit(+) | 96 ± 7 | − |
| NBM Lin(−)Sca-1(+)c-kit(+) | 415 ± 6 | + |
| 2-day 5 FU BM Lin(−)Sca-1(+)c-kit(+) | 194 ± 30 | + |
| NBM Lin(−)Sca-1(+)c-kit(+)CD34(+) | 15 ± 7 | ND |
| NBM Lin(−)Sca-1(+)c-kit(+)CD34(−) | 191 ± 64 | ND |
ND, not determined.
Hematopoietic progenitor cells were isolated from NBM or from 2d 5FU-treated BM of C57BL/6 mice as described in “Materials and Methods.” Sorted subpopulations were tested in various assays to establish their positions in the stem/progenitor cell differentiation pathway: HPP-CFC was assayed by incubating 100 to 500 cells in methylcellulose medium containing rmSF (50 ng/mL), rmIL-3 (20 ng/mL), rhIL-11 (50 ng/nL), and rhEPO (2 U/mL) for 14 days and by counting colonies with diameters greater than 2 mm. Lymphohematopoietic potential was defined as the ability of primary colonies grown for 8 to 12 days in SF + IL-11 to produce secondary colonies containing B220+ pre-B cells in rmSF + IL-7 supplemented methylcellulose cultures on replating. All results are expressed as mean ± SEM from 3 to 4 experiments.