Table 2. Inhibition of migration toward... | American Society of Hematology

Table 2.

Inhibition of migration toward vitronectin by HKa peptides

Protein	Domain	Sequence	Concentration of Peptide (μM)	Function in Molecule	% Inhibition at 0.2 μM	IC₅₀ μM
HK	D3	T255-Y280	139	Platelet binding site	74.0	>50
HK	D3	F-N334-V339-M	77	Hexapeptide from D3 + 15 random amino acids	5.0	>50
HK	GST-D5	K420-S513	0.2	Cell binding site	85.0^*	0.12
HK	D5	G406-N422	0.2	Endothelial cell binding site	19	>1
HK	D5	G440-H455	0.2	Neutrophil binding site	16	>1
HK	D5	H475-H485	0.2	Neutrophil binding site	12	>1
HK	D5	H483-G497	0.2	Endothelial cell binding site	12	>1
HK	D5	G486-K502	0.2	Heparin binding site	51^*	0.20
HK	D6	S565-P594	0.2	PK binding site	31^*	0.50

Protein	Domain	Sequence	Concentration of Peptide (μM)	Function in Molecule	% Inhibition at 0.2 μM	IC₅₀ μM
HK	D3	T255-Y280	139	Platelet binding site	74.0	>50
HK	D3	F-N334-V339-M	77	Hexapeptide from D3 + 15 random amino acids	5.0	>50
HK	GST-D5	K420-S513	0.2	Cell binding site	85.0^*	0.12
HK	D5	G406-N422	0.2	Endothelial cell binding site	19	>1
HK	D5	G440-H455	0.2	Neutrophil binding site	16	>1
HK	D5	H475-H485	0.2	Neutrophil binding site	12	>1
HK	D5	H483-G497	0.2	Endothelial cell binding site	12	>1
HK	D5	G486-K502	0.2	Heparin binding site	51^*	0.20
HK	D6	S565-P594	0.2	PK binding site	31^*	0.50

Sequences with inhibitory activity at less than 1 μM and greater than 20%. The results are the mean of 3 replicates from 1 of 2 similar experiments.